Optimization of Plant Regeneration in Different Pepper (Capsicum annuum L.) Lines
Autor: | Hülya Ilbi, Tolga Izgu, Yesim Yalcin Mendi |
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Přispěvatelé: | Ege Üniversitesi |
Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
organogenesis Organogenesis Biology 01 natural sciences 0-Belirlenecek Hypocotyl Crop lcsh:Agriculture callus Pepper plant growth regulators tissue culture lcsh:Agriculture (General) Regeneration (biology) [No Keywords] lcsh:S 04 agricultural and veterinary sciences General Medicine lcsh:S1-972 Capsicum annuum Horticulture Shoot 040103 agronomy & agriculture 0401 agriculture forestry and fisheries shoot formation 010606 plant biology & botany Explant culture |
Zdroj: | Turkish Journal of Agriculture: Food Science and Technology, Vol 8, Iss 2, Pp 471-477 (2020) |
Popis: | Development of an efficient plant regeneration protocol is essential for vegetable crop advancement by biotechnological methods. In this study, regeneration protocols of four pepper lines of different pepper types were optimized. Different protocols for organogenesis were investigated in regeneration experiments. Optimum plant regeneration was obtained in different combinations of 6-Benzylaminopurine (BAP) and Indole-3-acetic acid (IAA) in organogenesis assays. In organogenesis experiment, the highest shoot development was determined as 80% from hypocotyl explant of Demre pepper in 4 mg L-1 BA+0.5 mg L-1 IAA, 80% from hypocotyl explant of Charleston pepper in 3 mg L-1 BA+0.5 mg L-1 IAA, 80% from hypocotyl explant of capia pepper in 5 mg L-1 BA+0.5 mg L-1 and 84% from hypocotyl explant of bell pepper in 5 mg L-1 BA+0.5 mg L-1 IAA. Afterward, shoots were rooted and whole plants were obtained. |
Databáze: | OpenAIRE |
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