Replacement of the lysine residue in the consensus ATP‐binding sequence of the AddA subunit of AddAB drastically affects chromosomal recombination in transformation and transduction of Bacillus subtilis
| Autor: | Anne Hesseling, Jan Kooistra, Rob Meima, Bert Jan Haijema, Gerard Venema, Michiel A. Noback |
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| Přispěvatelé: | Groningen Biomolecular Sciences and Biotechnology |
| Rok vydání: | 1996 |
| Předmět: |
EXPRESSION
Exonucleases Exonuclease Enzyme complex DNA Repair DNA repair Protein subunit RECA PROTEIN ANALOG DEFICIENT MUTANTS Bacillus subtilis Microbiology HOMOLOGOUS RECOMBINATION CLONING Transduction (genetics) Adenosine Triphosphate Transformation Genetic Bacterial Proteins Transduction Genetic Consensus Sequence Molecular Biology Recombination Genetic Binding Sites CONSTRUCTION biology Lysine DNA Helicases Deoxyribonuclease biology.organism_classification Molecular biology Exodeoxyribonucleases Biochemistry ESCHERICHIA-COLI GENERAL-METHOD DNA-REPAIR Mutagenesis Site-Directed biology.protein bacteria DEPENDENT DEOXYRIBONUCLEASE Homologous recombination |
| Zdroj: | Molecular Microbiology, 21(5), 989-999. Wiley |
| ISSN: | 1365-2958 0950-382X |
| DOI: | 10.1046/j.1365-2958.1996.601424.x |
| Popis: | The ATP-dependent deoxyribonuclease enzyme complex (AddAB) of Bacillus subtilis possesses two consensus ATP-binding sequences, located in the N-terminal region of both subunits. The highly conserved lysine residues in both consensus ATP-binding sequences were replaced by glycine, resulting in the mutant enzyme complexes AddAB-A-K36G (AddA*B) and AddAB-B-K14G (AddAB*). The mutation in subunit AddA reduced DNA repair and chromosomal transformation, and abolished bacteriophage PBS1-mediated transduction. This mutation also resulted in a complete loss of the ATP-dependent exonuclease and helicase activity. In contrast, the mutation in subunit AddB had only marginal effects. The recF and addAB genes are not required for transformation with plasmid DNA, but have overlapping activities in transformation with chromosomal DNA. By contrast to RecF, the AddAB enzyme is essential for PBS1-mediated transduction. However, recF has a more important function with respect to DNA repair than addAB. |
| Databáze: | OpenAIRE |
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