The effectiveness of flow cytometric sorting of human sperm (MicroSort®) for influencing a child’s sex
| Autor: | Daniel A Potter, Joseph D. Schulman, Larry Johnson, Donald P. Marazzo, Marisa L Cole, David Karabinus, C. Opanga, Harvey J. Stern |
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| Rok vydání: | 2014 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Pregnancy Rate Semen Cell Separation Fertilization in Vitro Biology Insemination Miscarriage IUI Andrology Endocrinology Human fertilization ART procedures Pregnancy Human sperm sperm sorting medicine Humans Flow cytometry Prospective Studies Sex Preselection Sex Ratio Sperm Injections Intracytoplasmic In Situ Hybridization Fluorescence Insemination Artificial reproductive and urinary physiology Gynecology Chromosomes Human X Chromosomes Human Y Sex selection medicine.diagnostic_test urogenital system Research IVF/ICSI Infant Newborn Pregnancy Outcome FET Reproducibility of Results Obstetrics and Gynecology medicine.disease Spermatozoa Sperm Pregnancy rate Reproductive Medicine Female Developmental Biology Fluorescence in situ hybridization |
| Zdroj: | Reproductive Biology and Endocrinology : RB&E |
| ISSN: | 1477-7827 |
| DOI: | 10.1186/1477-7827-12-106 |
| Popis: | Background: Flow cytometric sorting can be used to separate sperm based on sex chromosome content. Differential fluorescence emitted by stained X- vs. Y-chromosome-bearing sperm enables sorting and collection of samples enriched in either X- or Y-bearing sperm for use to influence the likelihood that the offspring will be a particular sex. Herein we report the effectiveness of flow cytometric sorting of human sperm and its use in human ART procedures. Methods: This prospective, observational cohort study of the series of subjects treated with flow cytometrically sorted human sperm was conducted at investigational sites at two private reproductive centers. After meeting inclusion criteria, married couples (n = 4993) enrolled to reduce the likelihood of sex-linked or sex-limited disease in future children (n = 383) or to balance the sex ratio of their children (n = 4610). Fresh or frozen-thawed semen was processed and recovered sperm were stained with Hoechst 33342 and sorted by flow cytometry (n = 7718) to increase the percentage of X-bearing sperm (n = 5635) or Y-bearing sperm (n = 2083) in the sorted specimen. Sorted sperm were used for IUI (n = 4448) and IVF/ICSI (n = 2957). Measures of effectiveness were the percentage of X- and Y-bearing sperm in sorted samples, determined by fluorescence in situ hybridization, sex of babies born, IVF/ICSI fertilization- and cleavage rates, and IUI, IVF/ICSI, FET pregnancy rates and miscarriage rates. Results: Sorted specimens averaged 87.7 ± 5.0% X-bearing sperm after sorting for X and 74.3 ± 7.0% Y-bearing sperm after sorting for Y. Seventy-three percent of sorts were for girls. For babies born, 93.5% were females and 85.3% were males after sorting for X- and Y-bearing sperm, respectively. IUI, IVF/ICSI, and FET clinical pregnancy rates were 14.7%, 30.8%, and 32.1%, respectively; clinical miscarriage rates were 15.5%, 10.2%, and 12.7%. Conclusions: Flow cytometric sorting of human sperm shifted th eX :Y sperm ratio. IUI, IVF/ICSI and FET outcomes were consistent with unimpaired sperm function. Results provide evidence supporting the effectiveness of flow cytometric sorting of human sperm for use as a preconception method of influencing a baby’ ss ex. Trial registration: NCT00865735 (ClinicalTrials.gov) |
| Databáze: | OpenAIRE |
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