Monocyte culture and adherence modifies LPS-induced IL-6 production and inhibition by hydrocortisone
| Autor: | Donna M. Murasko, Walla L. Dempsey, Lisa M. Breuninger, I.Michael Goonewardene |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Lipopolysaccharides
medicine.medical_specialty Time Factors Lipopolysaccharide Hydrocortisone CD14 medicine.medical_treatment Immunology Stimulation Cell Communication Biology Pathology and Forensic Medicine chemistry.chemical_compound Mice Internal medicine medicine Cell Adhesion Immunology and Allergy Animals Humans Drug Interactions Cells Cultured Interleukin-6 Monocyte Kinetics medicine.anatomical_structure Cytokine Endocrinology Phenotype chemistry Cell culture Leukocytes Mononuclear Tumor necrosis factor alpha Glucocorticoid medicine.drug |
| Zdroj: | Clinical immunology and immunopathology. 72(2) |
| ISSN: | 0090-1229 |
| Popis: | The effect of hydrocortisone (HC) on IL-6 production by monocytes (Mo) that were cultured under either adherent or limited-adherent culture conditions was determined. Although freshly isolated Mo produced more IL-6 in response to LPS than Mo cultured 24 hr, culture had little effect on inhibition of IL-6 by HC. In contrast, the conditions of Mo culture had a significant impact on the kinetics of IL-6 production and inhibition by HC. Adherent Mo produced significantly higher levels of IL-6 4 hr after LPS compared to limited-adherent Mo. By 12 hr of LPS stimulation, comparable quantities of IL-6 were produced by both cultures. HC inhibition was complete 4 hr after LPS stimulation of adherent Mo, while 12 hr was required for maximum inhibition of limited-adherent Mo. Production of an IL-6 inhibitor was not responsible for the increased inhibition by HC with adherence, because both IL-6 protein and bioactivity were comparably decreased. The kinetics of inhibition of TNFα and IL-1β production by HC were similarly affected by adherence. Phenotypic analysis of cultured vs freshly isolated Mo showed a decrease in Mo expressing CD14, but not CD11b, which paralleled a decrease in IL-6 production by the cultured Mo. However, there were no phenotypic differences between adherent and limited-adherent Mo. The results demonstrate that the time of Mo culture, as well as adherence, may serve to alter LPS-induced IL-6 production and its inhibition by HC. |
| Databáze: | OpenAIRE |
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