Xenotransplantation of human fetal adipose tissue: a model of in vivo adipose tissue expansion and adipogenesis
| Autor: | Monique E. De Paepe, Stefan Kostadinov, Philip A. Gruppuso, Heather Francois-Vaughan, Omobola Onikoyi, Briana Garcia, Jennifer A. Sanders |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Fetal Tissue Transplantation obesity medicine.medical_specialty Transplantation Heterotopic Angiogenesis adipocytes Xenotransplantation medicine.medical_treatment Transplantation Heterologous Adipose tissue QD415-436 Mice SCID White adipose tissue Intra-Abdominal Fat Biology Kidney Models Biological Biochemistry angiogenesis chemistry.chemical_compound Endocrinology Pregnancy Adipocyte Internal medicine Methods medicine Animals Humans Adipogenesis Graft Survival Abortion Induced Cell Biology Stillbirth Subcutaneous Fat Abdominal Cell biology Transplantation nutrition Microscopy Fluorescence chemistry Pregnancy Trimester Second Female |
| Zdroj: | Journal of Lipid Research, Vol 55, Iss 12, Pp 2685-2691 (2014) |
| ISSN: | 0022-2275 |
| DOI: | 10.1194/jlr.d052787 |
| Popis: | Obesity during childhood and beyond may have its origins during fetal or early postnatal life. At present, there are no suitable in vivo experimental models to study factors that modulate or perturb human fetal white adipose tissue (WAT) expansion, remodeling, development, adipogenesis, angiogenesis, or epigenetics. We have developed such a model. It involves the xenotransplantation of midgestation human WAT into the renal subcapsular space of immunocompromised SCID-beige mice. After an initial latency period of approximately 2 weeks, the tissue begins expanding. The xenografts are healthy and show robust expansion and angiogenesis for at least 2 months following transplantation. Data and cell size and gene expression are consistent with active angiogenesis. The xenografts maintain the expression of genes associated with differentiated adipocyte function. In contrast to the fetal tissue, adult human WAT does not engraft. The long-term viability and phenotypic maintenance of fetal adipose tissue following xenotransplantation may be a function of its autonomous high rates of adipogenesis and angiogenesis. Through the manipulation of the host mice, this model system offers the opportunity to study the mechanisms by which nutrients and other environmental factors affect human adipose tissue development and biology. |
| Databáze: | OpenAIRE |
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