Characterization ofdnaJMultigene Family in the CyanobacteriumSynechococcus elongatusPCC 7942
| Autor: | Masumi Sato, Satoru Watanabe, Hikaru Yamahata, Kaori Nimura-Matsune, Hirofumi Yoshikawa |
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| Rok vydání: | 2007 |
| Předmět: |
endocrine system
Hot Temperature Synechococcus elongatus Blotting Western macromolecular substances Biology DNAJ Protein Applied Microbiology and Biotechnology Biochemistry Genome Analytical Chemistry DNA Algal Escherichia coli Luciferase DNAJB1 Molecular Biology Phylogeny DNA Primers Synechococcus Genetics Phylogenetic tree Reverse Transcriptase Polymerase Chain Reaction Algal Proteins Organic Chemistry General Medicine HSP40 Heat-Shock Proteins Up-Regulation Heat stress Multigene Family DNAJA2 Bacillus subtilis Plasmids Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 71:1021-1027 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.60691 |
| Popis: | The genome of the cyanobacterium Synechococcus elongatus PCC 7942 contains four dnaJ homologs, which are classified into three types based on domain structure. Among these, dnaJ1, dnaJ2, and dnaJ3 are essential for normal growth, and hence we analyzed them with a view to characterizing their specificity. Expression analysis indicated that dnaJ2, which encodes type II DnaJ protein, exhibited typical responses to heat and high-light stresses. Their localization and ability to prevent aggregation of luciferase were also diverse, suggesting a possible functional differentiation of these proteins. Since the expression of dnaJ1, which belongs to conserved type I DnaJ, down-regulated under heat stress, the unique structure of DnaJ2 may be involved in stress responses of S. elongatus. Based on phylogenetic analysis, the diverse dnaJ family was assumed to have evolved its own specific functions in each cyanobacterial species. |
| Databáze: | OpenAIRE |
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