Fluorometric immunoassay for human serum albumin based on its inhibitory effect on the immunoaggregation of quantum dots with silver nanoparticles
| Autor: | Vardan K. Gasparyan, Seda S. Marukhyan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Silver
Analytical chemistry Metal Nanoparticles Nanoparticle Serum Albumin Human 02 engineering and technology Conjugated system 010402 general chemistry 01 natural sciences Silver nanoparticle Analytical Chemistry Quantum Dots Fluorescence Resonance Energy Transfer medicine Humans Instrumentation Spectroscopy Immunoassay medicine.diagnostic_test Chemistry 021001 nanoscience & nanotechnology Human serum albumin Fluorescence Atomic and Molecular Physics and Optics 0104 chemical sciences body regions Förster resonance energy transfer Quantum dot embryonic structures Anisotropy 0210 nano-technology Nuclear chemistry medicine.drug |
| Zdroj: | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 173:34-38 |
| ISSN: | 1386-1425 |
| DOI: | 10.1016/j.saa.2016.08.029 |
| Popis: | Quantitative determination of HSA was conducted by competitive immunoassay. Inhibition of aggregation of antibody conjugated quantum dots (QD) with albumin conjugated silver nanoparticles (AgNPs) in the presence of HSA was conducted. If antibody-loaded CdSe QDs aggregate with HSA-coated silver nanoparticles the distance between the two kinds of nanoparticles will be reduced enough to cause fluorescence resonance energy transfer (FRET). In this case the yellow fluorescence of the Ab-QDs is quenched. However if HSA (antigen) is added to the Ab-QDs their surface will be blocked and they cannot aggregate any longer with the HSA-AgNPs. Hence, fluorescence will not be quenched. The drop of the intensity of fluorescence (peaking at 570nm) is inversely correlated with the concentration of HSA in the sample. The method allows to determine HSA in the 30-600ng·mL-1 concentration range. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect