The Drosophila splicing factor PSI is phosphorylated by casein kinase II and tousled-like kinase
| Autor: | Lori A. Kohlstaedt, Donald C. Rio, Daniela Mavrici, Julie L Aspden, Dhruv Marwha, Nathalie E. Cheng, J. Matthew Taliaferro |
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| Přispěvatelé: | Buratti, Emanuele |
| Rok vydání: | 2013 |
| Předmět: |
Proteomics
Exonic splicing enhancer lcsh:Medicine Biochemistry Mass Spectrometry 0302 clinical medicine Molecular Cell Biology Protein Interaction Mapping Signaling in Cellular Processes Drosophila Proteins Phosphorylation lcsh:Science Casein Kinase II 0303 health sciences Multidisciplinary Kinase Drosophila Melanogaster Nuclear Proteins RNA-Binding Proteins Animal Models Protein-Serine-Threonine Kinases Cell biology Nucleic acids Drosophila melanogaster RNA splicing Casein kinase 2 Biotechnology Research Article Signal Transduction General Science & Technology 1.1 Normal biological development and functioning RNA Splicing Biology Protein Serine-Threonine Kinases 03 medical and health sciences Splicing factor Model Organisms Underpinning research Protein splicing Genetics Animals Protein Interactions 030304 developmental biology lcsh:R Alternative splicing Proteins biology.organism_classification Molecular biology RNA processing Mutation RNA lcsh:Q Generic health relevance 030217 neurology & neurosurgery |
| Zdroj: | PLoS One, vol 8, iss 2 PLoS ONE PloS one, vol 8, iss 2 PLoS ONE, Vol 8, Iss 2, p e56401 (2013) |
| Popis: | Alternative splicing of pre-mRNA is a highly regulated process that allows cells to change their genetic informational output. These changes are mediated by protein factors that directly bind specific pre-mRNA sequences. Although much is known about how these splicing factors regulate pre-mRNA splicing events, comparatively little is known about the regulation of the splicing factors themselves. Here, we show that the Drosophila splicing factor P element Somatic Inhibitor (PSI) is phosphorylated at at least two different sites by at minimum two different kinases, casein kinase II (CK II) and tousled-like kinase (tlk). These phosphorylation events may be important for regulating protein-protein interactions involving PSI. Additionally, we show that PSI interacts with several proteins in Drosophila S2 tissue culture cells, the majority of which are splicing factors. |
| Databáze: | OpenAIRE |
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