From structure and functions of steroidogenic enzymes to new technologies of gene engineering
| Autor: | Stephan Mauersberger, Ya. V. Faletrov, Valentin N. Luzikov, I. E. Kovaleva, Vladimir M. Shkumatov, L.A. Novikova |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Recombinant Fusion Proteins
Transgene Saccharomyces cerevisiae Biology medicine.disease_cause Biochemistry Fungal Proteins Bacterial Proteins Cytochrome P-450 Enzyme System medicine Animals Humans Cholesterol Side-Chain Cleavage Enzyme Escherichia coli chemistry.chemical_classification Bacteria Molecular Structure Organisms Genetically Modified Cholesterol side-chain cleavage enzyme Cytochrome P450 Yarrowia General Medicine biology.organism_classification Mitochondria Transformation (genetics) Enzyme chemistry Drug Design Microsomes Liver biology.protein Steroids Genetic Engineering |
| Zdroj: | Biochemistry (Moscow). 74:1482-1504 |
| ISSN: | 1608-3040 0006-2979 |
| DOI: | 10.1134/s0006297909130057 |
| Popis: | (3/4) This review summarizes data about structural and functional organization of steroidogenic P450-dependent enzymatic systems. Problems of catalysis of steroid substrate transformation, special features of mitochondrial type P450scc topogenesis, and abilities of some microbial electron transport proteins to support P450 activity in vitro and in vivo are considered. Principal steps in the creation and catalytic properties of transgenic strains of Escherichia coli, Saccharomyces cerevisiae, and Yarrowia lipolytica expressing both mammalian steroidogenic P450s and the corresponding electron transport proteins are also described. Achievements and prospects of using such transgenic strains for biotechnological synthesis and pharmacological screening are considered. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|