Interactive Effects of Light and Melatonin on Biosynthesis of Silymarin and Anti-Inflammatory Potential in Callus Cultures of Silybum marianum (L.) Gaertn
| Autor: | Duangjai Tungmunnithum, Muhammad Asad Ullah, Muhammad Zeeshan Younas, Christophe Hano, Muzamil Shah, Nathalie Giglioli-Guivarc'h, Samantha Drouet, Bilal Haider Abbasi |
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| Přispěvatelé: | Quaid-i-Azam University (QAU), Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), Université d'Orléans (UO)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), COSM'ACTIFS - Bioactifs et Cosmétiques, Université d'Orléans (UO)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Mahidol University [Bangkok], Biomolécules et biotechnologies végétales (BBV EA 2106), Université de Tours, Université de Tours (UT) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
mélatonine
0106 biological sciences Antioxidant antioxidant silymarin medicine.medical_treatment [SDV]Life Sciences [q-bio] Flavonoid Pharmaceutical Science melatonin effet de la lumière 01 natural sciences Analytical Chemistry antiinflammatoire Drug Discovery Food science anti-inflammatory photoperiodism chemistry.chemical_classification 0303 health sciences culture cellulaire biology Chemistry activité antioxydante anti-inflammatoire Silybum marianum (L.) Gaertn plante médicinale Phytochemical Chemistry (miscellaneous) Molecular Medicine medicine.drug medicine.drug_class Silybum marianum (L phenolics composé phénolique Biotechnologies activité anti-oxydante Anti-inflammatory Silybum marianum lcsh:QD241-441 Melatonin 03 medical and health sciences antioxydant lcsh:Organic chemistry light regimes medicine Physical and Theoretical Chemistry activité anti-inflammatoire 030304 developmental biology Organic Chemistry luminosité ) Gaertn flavonoids biology.organism_classification métabolite secondaire Callus 010606 plant biology & botany |
| Zdroj: | Molecules Molecules, MDPI, 2019, 24 (7), pp.1207. ⟨10.3390/molecules24071207⟩ Molecules, Vol 24, Iss 7, p 1207 (2019) Molecules 7 (24), . (2019) Volume 24 Issue 7 |
| ISSN: | 1420-3049 |
| Popis: | Silybum marianum (L.) Gaertn. is a well-known medicinal herb, primarily used in liver protection. Light strongly affects several physiological processes along with secondary metabolites biosynthesis in plants. Herein, S. marianum was exploited for in vitro potential under different light regimes in the presence of melatonin. The optimal callogenic response occurred in the combination of 1.0 mg/L &alpha naphthalene acetic acid and 0.5 mg/L 6-benzylaminopurine under photoperiod. Continuous light associated with melatonin treatment increased total flavonoid content (TFC), total phenolic content (TPC) and antioxidant potential, followed by photoperiod and dark treatments. The increased level of melatonin has a synergistic effect on biomass accumulation under continuous light and photoperiod, while an adverse effect was observed under dark conditions. More detailed phytochemical analysis showed maximum total silymarin content (11.92 mg/g dry weight (DW)) when placed under continuous light + 1.0 mg/L melatonin. Individually, the level of silybins (A and B), silydianin, isolsilychristin and silychristin was found highest under continuous light. Anti-inflammatory activities were also studied and highest percent inhibition was recorded against 15-lipoxygenase (15-LOX) for cultures cultivated under continuous light (42.33%). The current study helps us to better understand the influence of melatonin and different light regimes on silymarin production as well as antioxidant and anti-inflammatory activities in S. marianum callus extracts. |
| Databáze: | OpenAIRE |
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