Lycium barbarum Polysaccharide Suppresses Expression of Fibrotic Proteins in Primary Human Corneal Fibroblasts
| Autor: | Louis Tong, Tommy C Y Chan, Alex Lap-Ki Ng, Yashan Bu, Vishal Jhanji, Sum Sum Kwok, Francisca S. Y. Wong, Yau Kei Chan, Amy C. Y. Lo, Gary Hin-Fai Yam, Kendrick Co Shih |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
genetic structures
lcsh:Medicine Immunofluorescence Article fibroblast Extracellular matrix 03 medical and health sciences 0302 clinical medicine Western blot Fibrosis health services administration Gene expression Medicine Viability assay human keratocytes Fibroblast corneal scarring 030304 developmental biology 0303 health sciences Lycium barbarum polysaccharides biology medicine.diagnostic_test business.industry lcsh:R TGFβ1 General Medicine Transforming growth factor beta pathological conditions signs and symptoms medicine.disease Molecular biology nervous system diseases body regions medicine.anatomical_structure 030221 ophthalmology & optometry biology.protein population characteristics business |
| Zdroj: | Journal of Clinical Medicine Volume 9 Issue 11 Journal of Clinical Medicine, Vol 9, Iss 3572, p 3572 (2020) |
| ISSN: | 2077-0383 |
| DOI: | 10.3390/jcm9113572 |
| Popis: | (1) Objective: To study the anti-fibrotic effects of Lycium barbarum polysaccharides (LBP) on corneal stromal fibroblasts and assess LBP&rsquo s effect on cell viability. (2) Methods: Primary human corneal keratocytes of passage 3 to 6 were used for all experiments. Cells are pretreated with LBP solution for 24 h and then transforming growth factor beta 1 (TGF&beta 1) for 48 h and collected for experiments. Fibrotic protein analysis was performed using immunofluorescence and Western blot. The effect of LBP on cell viability was assessed using the MTS assay. (3) Results: LBP significantly reduced the expression of fibrotic proteins, including &alpha SMA and extracellular matrix proteins (collagen type I and III). LBP significantly decreased the viability of myofibroblasts but not the fibroblasts. Conclusions: In this study, LBP was effective in the prevention of fibrosis gene expression. Further studies to assess the underlying mechanism and pharmacological properties will facilitate the formation of a topical LBP solution for in vivo studies. |
| Databáze: | OpenAIRE |
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