S100-A10, thioredoxin, and S100-A6 as biomarkers of papillary thyroid carcinoma with lymph node metastasis identified by MALDI Imaging
| Autor: | Hakan Sarioglu, Benjamin Balluff, Martin Nipp, Heinz Höfler, Axel Walch, Marius Ueffing, Sandra Rauser, Horst Zitzelsberger, Kristian Unger, Mareike Elsner, Stephan Meding |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
MALDI imaging
Adult Male Pathology medicine.medical_specialty Biology Mass spectrometry imaging Papillary thyroid cancer Metastasis Thioredoxins Drug Discovery Carcinoma medicine Biomarkers Tumor Humans Thyroid Neoplasms Neoplasm Metastasis Thyroid cancer Genetics (clinical) Neoplasm Staging Tissue microarray S100 Proteins Middle Aged medicine.disease Primary tumor Carcinoma Papillary Gene Expression Regulation Neoplastic Thyroid Cancer Papillary Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Molecular Medicine Female Lymph Nodes S100-A10 S100-A6 Thioredoxin |
| Zdroj: | J. Mol. Med. 90, 163-174 (2012) |
| Popis: | In papillary thyroid carcinoma (PTC), metastasis is a feature of an aggressive tumor phenotype. To identify protein biomarkers that distinguish patients with an aggressive tumor behavior, proteomic signatures in metastatic and non-metastatic tumors were investigated comparatively. In particular, matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) was used to analyze primary tumor samples. We investigated a tumor cohort of PTC (n = 118) that were matched for age, tumor stage, and gender. Proteomic screening by MALDI-IMS was performed for a discovery set (n = 29). Proteins related to the discriminating mass peaks were identified by 1D-gel electrophoresis followed by mass spectrometry. The candidate proteins were subsequently validated by immunohistochemistry (IHC) using a tissue microarray for an independent PTC validation set (n = 89). In this study, we found 36 mass-to-charge-ratio (m/z) species that specifically distinguished metastatic from non-metastatic tumors, among which m/z 11,608 was identified as thioredoxin, m/z 11,184 as S100-A10, and m/z 10,094 as S100-A6. Furthermore, using IHC on the validation set, we showed that the overexpression of these three proteins was highly associated with lymph node metastasis in PTC (p&thinsp |
| Databáze: | OpenAIRE |
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