Astrovirus infection in hospitalized infants with severe combined immunodeficiency after allogeneic hematopoietic stem cell transplantation
| Autor: | Rosmarie Caduff, Samuel Cordey, Tayfun Guengoer, Thomas Alexander Mckee, Alexandra Trkola, Walter Bossart, Werner Wunderli, Sandra Van Belle, Laurent Kaiser, Caroline Tapparel, Astrid Meerbach, Manuel Schibler, Oliver Greiner, Daniel Gerlach, Christoph Berger |
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| Přispěvatelé: | University of Zurich, Wunderli, W |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
10028 Institute of Medical Virology
Male Viral Diseases medicine.medical_treatment lcsh:Medicine Hematopoietic stem cell transplantation ddc:616.07 Transplantation Homologous/adverse effects Pediatrics Astroviridae Infections/diagnosis/mortality/virology Astroviridae Infections Astrovirus Infection lcsh:Science Pathogen Immune Response Cytopathic effect ddc:616 0303 health sciences Multidisciplinary Stem Cells Hematopoietic Stem Cell Transplantation Meningoencephalitis 3. Good health RNA Viral/genetics Infectious Diseases Medicine RNA Viral Research Article Infectious Disease Control Immunology Viremia 610 Medicine & health 1100 General Agricultural and Biological Sciences Biology Real-Time Polymerase Chain Reaction Astrovirus 03 medical and health sciences 1300 General Biochemistry Genetics and Molecular Biology 10049 Institute of Pathology and Molecular Pathology medicine Transplantation Homologous Humans Severe Combined Immunodeficiency/mortality/therapy/virology Hematopoietic Stem Cell Transplantation/adverse effects Embryonic Stem Cells 030304 developmental biology Retrospective Studies Severe combined immunodeficiency 1000 Multidisciplinary 030306 microbiology lcsh:R Infant medicine.disease biology.organism_classification Virology 10036 Medical Clinic Immune System 570 Life sciences biology Severe Combined Immunodeficiency lcsh:Q Caco-2 Cells Developmental Biology |
| Zdroj: | PLOS ONE, Vol. 6, No 11 (2011) P. e27483 PloS one PLoS ONE, Vol 6, Iss 11, p e27483 (2011) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Infants with severe primary combined immunodeficiency (SCID) and children post-allogeneic hematopoietic stem cell transplantation (HSCT) are extremely susceptible to unusual infections. The lack of generic tools to detect disease-causing viruses among more than 200 potential human viral pathogens represents a major challenge to clinicians and virologists. We investigated retrospectively the causes of a fatal disseminated viral infection with meningoencephalitis in an infant with gamma C-SCID and of chronic gastroenteritis in 2 other infants admitted for HSCT during the same time period. Analysis was undertaken by combining cell culture, electron microscopy and sequence-independent single primer amplification (SISPA) techniques. Caco-2 cells inoculated with fecal samples developed a cytopathic effect and non-enveloped viral particles in infected cells were detected by electron microscopy. SISPA led to the identification of astrovirus as the pathogen. Both sequencing of the capsid gene and the pattern of infection suggested nosocomial transmission from a chronically excreting index case to 2 other patients leading to fatal infection in 1 and to transient disease in the others. Virus-specific, real-time reverse transcription polymerase chain reaction was then performed on different stored samples to assess the extent of infection. Infection was associated with viremia in 2 cases and contributed to death in 1. At autopsy, viral RNA was detected in the brain and different other organs, while immunochemistry confirmed infection of gastrointestinal tissues. This report illustrates the usefulness of the combined use of classical virology procedures and modern molecular tools for the diagnosis of unexpected infections. It illustrates that astrovirus has the potential to cause severe disseminated lethal infection in highly immunocompromised pediatric patients. |
| Databáze: | OpenAIRE |
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