Static stretch affects neural stem cell differentiation in an extracellular matrix-dependent manner
| Autor: | James C. Earthman, Janahan Arulmoli, Francesco Tombola, Lisa P. McDonnell, Lisa A. Flanagan, Medha M. Pathak, Jamison L. Nourse |
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| Rok vydání: | 2015 |
| Předmět: |
Integrins
Cellular differentiation Cells 1.1 Normal biological development and functioning Integrin Stress Regenerative Medicine Article Extracellular matrix 03 medical and health sciences Mice 0302 clinical medicine Neural Stem Cells Underpinning research Animals Progenitor cell Mechanotransduction Cells Cultured 030304 developmental biology 0303 health sciences Multidisciplinary Cultured biology Chemistry Neurosciences Cell Differentiation Mechanical Stem Cell Research Neural stem cell Cell biology Extracellular Matrix Brain Disorders Fibronectin Transplantation Other Physical Sciences Oligodendroglia biology.protein Stress Mechanical Laminin Biochemistry and Cell Biology 030217 neurology & neurosurgery Protein Binding |
| Zdroj: | Scientific reports, vol 5, iss 1 Arulmoli, J; Pathak, MM; McDonnell, LP; Nourse, JL; Tombola, F; Earthman, JC; et al.(2015). Static stretch affects neural stem cell differentiation in an extracellular matrix-dependent manner. Scientific Reports, 5. doi: 10.1038/srep08499. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/5599n63w Scientific Reports |
| DOI: | 10.1038/srep08499. |
| Popis: | Neural stem and progenitor cell (NSPC) fate is strongly influenced by mechanotransduction as modulation of substrate stiffness affects lineage choice. Other types of mechanical stimuli, such as stretch (tensile strain), occur during CNS development and trauma, but their consequences for NSPC differentiation have not been reported. We delivered a 10% static equibiaxial stretch to NSPCs and examined effects on differentiation. We found static stretch specifically impacts NSPC differentiation into oligodendrocytes, but not neurons or astrocytes and this effect is dependent on particular extracellular matrix (ECM)-integrin linkages. Generation of oligodendrocytes from NSPCs was reduced on laminin, an outcome likely mediated by the α6 laminin-binding integrin, whereas similar effects were not observed for NSPCs on fibronectin. Our data demonstrate a direct role for tensile strain in dictating the lineage choice of NSPCs and indicate the dependence of this phenomenon on specific substrate materials, which should be taken into account for the design of biomaterials for NSPC transplantation. |
| Databáze: | OpenAIRE |
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