The multienzymatic protein CAD leading the de novo biosynthesis of pyrimidines localizes exclusively in the cytoplasm and does not translocate to the nucleus
| Autor: | Santiago Ramón-Maiques, Francisco Del Caño-Ochoa |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Cytoplasm
Active Transport Cell Nucleus Chromosomal translocation 010402 general chemistry 01 natural sciences Biochemistry Cell Line Aspartate Carbamoyltransferase Genetics medicine Humans cardiovascular diseases Dihydroorotase Cell Nucleus 010405 organic chemistry Chemistry Cell growth General Medicine Subcellular localization 0104 chemical sciences Cell biology Cytosol Pyrimidines medicine.anatomical_structure Molecular Medicine Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) Cell fractionation Nucleus |
| Zdroj: | Nucleosides, Nucleotides & Nucleic Acids. 39:1320-1334 |
| ISSN: | 1532-2335 1525-7770 |
| Popis: | CAD, the multienzymatic protein that initiates and controls the de novo biosynthesis of pyrimidines, plays a major role in nucleotide homeostasis, cell growth and proliferation. Despite its interest as a potential antitumoral target, there is a lack of understanding on CAD's structure and functioning mechanisms. Although mainly identified as a cytosolic complex, different studies support the translocation of CAD into the nucleus, where it could have a yet undefined function. Here, we track the subcellular localization of CAD by using fluorescent chimeras, cell fractionation and immunoblotting with specific antibodies. Contradicting previous studies, we demonstrate that CAD is exclusively localized at the cytosol and discard a possible translocation to the nucleus. |
| Databáze: | OpenAIRE |
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