Decidualization score identifies an endometrial dysregulation in samples from women with recurrent pregnancy losses and unexplained infertility
| Autor: | Alfredo Germain, Svetlana Dambaeva, Kenneth D. Beaman, Sylvia Schneiderman, Emilio Fernandez, Joanne Kwak-Kim, Mahmood Bilal, Carolyn Coulam |
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| Rok vydání: | 2021 |
| Předmět: |
Gynecology
Infertility medicine.medical_specialty Pregnancy recurrent pregnancy loss medicine.diagnostic_test business.industry Reproductive medicine Decidualization Retrospective cohort study lcsh:Diseases of the genitourinary system. Urology lcsh:RC870-923 medicine.disease lcsh:Gynecology and obstetrics Endometrium decidualization gene expression medicine Original Article infertility business lcsh:RG1-991 Tissue homeostasis Endometrial biopsy Unexplained infertility |
| Zdroj: | F&S Reports F&S Reports, Vol 2, Iss 1, Pp 95-103 (2021) |
| ISSN: | 2666-3341 |
| DOI: | 10.1016/j.xfre.2020.12.004 |
| Popis: | Objective: To study decidualization-associated endometrial factors. Design: Retrospective cohort study to compare endometrial gene expression patterns in women experiencing reproductive failure including recurrent pregnancy loss or unexplained infertility versus fertile controls. Setting: University Reproductive Medicine Center. Patient(s): Women experiencing recurrent reproductive failure including recurrent pregnancy loss or unexplained infertility (n = 42) and fertile controls (n = 18). Intervention(s): Endometrial biopsy samples were analyzed with targeted ribonucleic acid sequencing via next-generation sequencing. Main Outcome Measure(s): The primary end point measurements were the expression of genes important for endometrial transformation during decidualization measured singly and in a combined/cumulative score approach. The secondary end point measurements were receiver operating curve analysis and comparisons between the specific biomarkers. Result(s): The comparison revealed differential expression of factors associated with decidualization, tissue homeostasis, and immune regulation: FOXO1, GZMB, IL15, SCNN1A, SGK1, and SLC2A1. A combined evaluation of these 6 signature factors was designated as a decidualization score in which the maximal score was “6” and the minimal was “0”. Among controls, 89% of the samples had a score ≥5 and 11% had a score of “4”. A total of 76% of samples in the patient group had scores ≤4 and 19% had the lowest score of “0”. A decidualization score |
| Databáze: | OpenAIRE |
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