Methods for the analysis of cellular kinetics in PHA-stimulated blood lymphocytes using BrdU incorporation. A comparative study
| Autor: | V. Palma, L. Buentello, F. Salamanca, H. Tudón, P. Ostrosky, S. Nava |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Adolescent genetic structures Health Toxicology and Mutagenesis Lymphocyte Cytological Techniques Kinetics Chromatids Cycle time Genetics medicine Humans Lymphocytes Phytohemagglutinins Child Molecular Biology Cells Cultured Generation time Cell growth Chemistry Cell Cycle Infant Cell cycle Cellular kinetics Cell cycle analysis medicine.anatomical_structure Bromodeoxyuridine Biochemistry Child Preschool Female Biological system Cell Division |
| Zdroj: | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 286:267-273 |
| ISSN: | 0027-5107 |
| DOI: | 10.1016/0027-5107(93)90191-h |
| Popis: | The cellular cycle (cc) span was measured by using differential sister-chromatid staining (DSCS) and applying the following methods: cellular cycle time (cct) according to the graphic method of Dutrillaux and Fosse (G-cct); the analytical equation (A-cct) proposed in the present paper, and the average generation time (AGT) suggested by Ivett and Tice. The mean values obtained by the three methods were 12.5, 12.7, and 19.5 h, respectively. A-cct is the more precise method, since the equation of the analytical procedure allows the utilization of numerical data, and when the graphical method is used, the values plotted in a graph may vary according to the employed scale. Cct is the choice over AGT because the first evaluates actively dividing cells and only considers those at M2 or M3. It will be useful to study cell proliferation kinetics in genetic pathological conditions and to investigate with accuracy the effect of cytostatic and cytotoxic drugs. |
| Databáze: | OpenAIRE |
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