How to broaden enzyme substrate specificity: strategies, implications and applications
| Autor: | Jean-Luc Jestin, Sophie Vichier-Guerre |
|---|---|
| Přispěvatelé: | Chimie Organique, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Chimie Organique (UCO), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Biology
MESH: Thermus 01 natural sciences Microbiology Genome MESH: DNA Nucleotidylexotransferase Catalysis Substrate Specificity 03 medical and health sciences DNA Nucleotidylexotransferase MESH: Directed Molecular Evolution Thermus Molecular Biology DNA-directed DNA polymerase 030304 developmental biology chemistry.chemical_classification 0303 health sciences 010405 organic chemistry DNA-Directed RNA Polymerases General Medicine MESH: Catalysis 0104 chemical sciences MESH: DNA-Directed RNA Polymerases Enzyme chemistry Biochemistry Substrate specificity MESH: Substrate Specificity Directed Molecular Evolution |
| Zdroj: | Research in Microbiology Research in Microbiology, 2005, 156 (10), pp.961-6. ⟨10.1016/j.resmic.2005.09.004⟩ Research in Microbiology, Elsevier, 2005, 156 (10), pp.961-6. ⟨10.1016/j.resmic.2005.09.004⟩ |
| ISSN: | 0923-2508 |
| DOI: | 10.1016/j.resmic.2005.09.004⟩ |
| Popis: | For identification of mutations associated with the broadening of enzyme substrate specificity, three strategies, including directed enzyme evolution, are described for selected examples. Implications concerning enzyme models are highlighted. Applications to the field of biocatalysis are discussed. A bidimensional map for the classification of enzyme activities is suggested so as to improve genome annotations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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