Mechanics of interstitial-lymphatic fluid transport: theoretical foundation and experimental validation

Autor: Melody A. Swartz, Paolo A. Netti, Alan J. Grodzinsky, Yves Boucher, Rakesh K. Jain, Christian Brekken, Arja Kaipainen
Přispěvatelé: Swartz, M. A., Kaipainen, A., Netti, PAOLO ANTONIO, Brekken, C., Boucher, Y., Grodzinsky, A. J., Jain, R. K.
Rok vydání: 1999
Předmět:
Skin Physiology
Tissue fluid
Nude
Lymph/*physiology
Mice
Hydraulic conductivity
Skin Physiological Phenomena
Edema
Biomechanics
Orthopedics and Sports Medicine
Skin
Microscopy
Chemistry
Rehabilitation
Dextrans
Mechanics
Water-Electrolyte Balance
Biomechanical Phenomena
Lymphatic system
Female
Lymph
medicine.symptom
Swelling
Fluorescein-5-isothiocyanate
Tail
Models
Poromechanics
Biomedical Engineering
Biophysics
Mice
Nude
Fluorescein-5-isothiocyanate/analogs & derivatives
In Vitro Techniques
Models
Biological
Fluorescence
Lymphatic System
Skin/anatomy & histology
Interstitial fluid
Pressure
medicine
Animals
Lymphatic System/anatomy & histology/physiology
Biological
Edema/physiopathology
Elasticity
Microscopy
Fluorescence
Extracellular Space/*physiology
Water-Electrolyte Balance/*physiology
Extracellular Space
Zdroj: Journal of Biomechanics. 32:1297-1307
ISSN: 0021-9290
DOI: 10.1016/s0021-9290(99)00125-6
Popis: Interstitial fluid movement is intrinsically linked to lymphatic drainage. However, their relationship is poorly understood, and associated pathologies are mostly untreatable. In this work we test the hypothesis that bulk tissue fluid movement can be evaluated in situ and described by a linear biphasic theory which integrates the regulatory function of the lymphatics with the mechanical stresses of the tissue. To accomplish this, we develop a novel experimental and theoretical model using the skin of the mouse tail. We then use the model to demonstrate how interstitial-lymphatic fluid movement depends on a balance between the elasticity, hydraulic conductivity, and lymphatic conductance as well as to demonstrate how chronic swelling (edema) alters the equipoise between tissue fluid balance parameters. Specifically, tissue fluid equilibrium is perturbed with a continuous interstitial infusion of saline into the tip of the tail. The resulting gradients in tissue stress are measured in terms of interstitial fluid pressure using a servo-null system. These measurements are then fit to the theory to provide in vivo estimates of the tissue hydraulic conductivity, elastic modulus, and overall resistance to lymphatic drainage. Additional experiments are performed on edematous tails to show that although chronic swelling causes an increase in the hydraulic conductivity, its greatly increased distensibility (due to matrix remodeling) dampens the driving forces for fluid movement and leads to fluid stagnation. This model is useful for examining potential treatments for edema and lymphatic disorders as well as substances which may alter tissue fluid balance and/or lymphatic drainage.
Databáze: OpenAIRE
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