Clinical utility of microarray B‐cell epitope mapping in food allergies: A systematic review
| Autor: | Belén de la Hoz, Laura Sánchez-Ruano, Javier Martínez-Botas |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Microarray
Wilcoxon signed-rank test Immunology Bioinformatics Epitope 03 medical and health sciences 0302 clinical medicine Immunologic Technique Food allergy medicine Animals Humans Immunology and Allergy 030212 general & internal medicine business.industry Reproducibility of Results Allergens Microarray Analysis medicine.disease Precision medicine Epitope mapping 030228 respiratory system Food Pediatrics Perinatology and Child Health Epitopes B-Lymphocyte Peptide microarray Peptides business Epitope Mapping Food Hypersensitivity |
| Zdroj: | Pediatric Allergy and Immunology. 31:175-185 |
| ISSN: | 1399-3038 0905-6157 |
| DOI: | 10.1111/pai.13141 |
| Popis: | Background Peptide microarray technology has been proposed as a useful tool for diagnosing food allergy. However, there is considerable heterogeneity in the clinical methods and analytical procedures used to assess its diagnostic and prognostic performance. We performed a systematic review of studies that have used B-cell epitopes by peptide microarray in food allergies to identify the clinical utility of this immunologic technique. Methods Studies were screened in PubMed, Web of Science, and Embase according to an established keyword algorithm. Data extraction was performed, and information was collected in an Excel database. Descriptive analysis was carried out using Stata software. Results Thirty relevant studies were identified. Most articles were cross-sectional (n = 24), included epitope mapping (n = 9), and assessed diagnostic utility (n = 11). All studies recruited allergic patients, and some included additional patients (sensitized, persistent, and tolerant). The primary microarray variables studied were IgE intensity (n = 29), IgG4 intensity (n = 15), and number of peptides (n = 17). Statistical approaches differed significantly between studies, with the Wilcoxon test being the most frequently used (n = 10). Conclusions Sensitization to particular epitopes of milk, peanut, and shrimp allergens can be used to determine clinical reactivity, persistence, severity, or response to oral immunotherapy; however, important methodological questions need to be addressed before drawing definitive conclusions. More research is needed to address the accuracy and clinical benefits of microarray-based technology. Standards are required to improve consistency and reproducibility, and to allow for better understanding of research findings. |
| Databáze: | OpenAIRE |
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