Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells
| Autor: | Nicole Westerfeld, Sophie Nallet, Lucia Baldi, Iwo König, David L. Hacker, Mario Amacker, Christiane Zaborosch, Rinaldo Zurbriggen, Florian M. Wurm |
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| Rok vydání: | 2009 |
| Předmět: |
Technology
Recombinant protein Influenza Virosomes Virosomes Recombinant Fusion Proteins Protein subunit Respiratory Syncytial Virus Infections Biology Antibodies Viral Transfection Virus Cell Line law.invention RSV vaccine 615: Pharmakologie und Therapeutik Mice Transient transfection Neutralization Tests Immunity law Respiratory Syncytial Virus Vaccines Animals Humans Children Viral antibodies Delivery-System Purification Mice Inbred BALB C General Veterinary General Immunology and Microbiology Subunit vaccines Public Health Environmental and Occupational Health Inbred BALB C mice Virology Fusion protein Vaccination Efficient Infectious Diseases Cell culture Vaccines Subunit Recombinant DNA Molecular Medicine Infection Viral Fusion Proteins Large-Scale Production |
| Zdroj: | Vaccine. 27:6415-6419 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2009.06.019 |
| Popis: | Although respiratory syncytial virus (RSV) causes severe lower respiratory tract infection in infants and adults at risk, no RSV vaccine is currently available. In this report, efforts toward the generation of an RSV subunit vaccine using recombinant RSV fusion protein (rRSV-F) are described. The recombinant protein was produced by transient gene expression (TGE) in suspension-adapted human embryonic kidney cells (HEK-293E) in 4 L orbitally shaken bioreactors. It was then purified and formulated in immunostimulating reconstituted influenza virosomes (IRIVs). The candidate vaccine induced anti-RSV-F neutralizing antibodies in mice, and challenge studies in cotton rats are ongoing. If successful in preclinical and clinical trials, this will be the first recombinant subunit vaccine produced by large-scale TGE in mammalian cells. |
| Databáze: | OpenAIRE |
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