Glycomimetic affinity-enrichment proteomics identifies partners for a clinically-utilized iminosugar

Autor:	Sarah E. Lloyd, Benjamin G. Davis, Frances M. Platt, Aarnoud C. van der Spoel, C. Celeste Chuang, Isa N. Cruz, Holger B. Kramer, Conor S. Barry, Min Yang
Rok vydání:	2013
Předmět:	chemistry.chemical_classification biology Glycoconjugate Iminosugar General Chemistry Computational biology Proteomics Article Mammalian tissue chemistry Biochemistry Glycomimetic Male fertility Chaperone (protein) biology.protein Repurposing
Zdroj:	Chemical Science. 4:3442
ISSN:	2041-6539 2041-6520
DOI:	10.1039/c3sc50826a
Popis:	Widescale evaluation of interacting partners for carbohydrates is an underexploited area. Probing of the 'glyco-interactome' has particular relevance given the lack of direct genetic control of glycoconjugate biosynthesis. Here we design, create and utilize a natural product-derived glycomimetic iminosugar probe in a Glycomimetic Affinity-enrichment Proteomics (Glyco-AeP) strategy to elucidate key interactions directly from mammalian tissue. The binding partners discovered here and the associated genomic analysis implicate a subset of chaperone and junctional proteins as important in male fertility. Such repurposing of existing therapeutics thus creates direct routes to probing in vivo function. The success of this strategy suggests a general approach to discovering 'carbohydrate-active' partners in biology. © 2013 The Royal Society of Chemistry.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d8fecfc3fd0f6bb6dbd253e6fe334de https://doi.org/10.1039/c3sc50826a Zobrazit plný text záznamu