Genome-wide gene expression differences in Crohnʼs disease and ulcerative colitis from endoscopic pinch biopsies: Insights into distinctive pathogenesis
| Autor: | Theodore M. Bayless, Shukti Chakravarti, Themistocles Dassopoulos, Mary L. Harris, Leslie Cope, Anirban Maitra, Giovanni Parmigiani, Feng Wu, Steven R. Brant |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male Biopsy Gene Expression Biology Infectious Colitis Inflammatory bowel disease Crohn Disease Gene expression medicine Cluster Analysis Humans Immunology and Allergy Intestine Large Colitis Aged Oligonucleotide Array Sequence Analysis Aged 80 and over Crohn's disease Genome Human Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Gastroenterology Endoscopy Middle Aged medicine.disease Ulcerative colitis digestive system diseases Gene expression profiling Immunology Cancer research Colitis Ulcerative Female TAP1 |
| Zdroj: | Inflammatory Bowel Diseases. 13:807-821 |
| ISSN: | 1078-0998 |
| Popis: | Background: Ulcerative colitis (UC) and Crohn's disease (CD) are inflammatory bowel diseases (IBD) with variable, overlapping clinical features and complex pathophysiologies. Methods: To identify pathogenic processes underlying these disease subtypes, we used single endoscopic pinch biopsies to elucidate patterns of gene expression in active and inactive areas of UC and CD and compared these to infectious colitis and healthy control samples. Results: Unsupervised classification of a total of 36 samples yielded promising separation between the affected IBD, unaffected IBD, non-IBD colitis, and normal control samples, suggesting each sample type had a distinctive gene expression pattern. Genes differentially expressed in the CD samples compared to in the controls were related to IFNγ-inducible TH1 processes (IFITM1, IFITM3, STAT1, and STAT3) and antigen presentation (TAP1, PSME2, PSMB8). The most noticeable change in the UC samples was reduced expression of genes regulating biosynthesis, metabolism, and electrolyte transport (HNF4G, KLF5, AQP8, ATP2B1, and SLC16A). Twenty-five percent of genes down-regulated in the UC samples were also down-regulated in the infectious colitis samples. Unaffected biopsy samples of IBD patients also registered differences expression of genes compared to in the normal controls. Of these differentially expressed genes, only 2 were up-regulated, PSKH1, a regulator of mRNA processing, and PPID, a suppressor of apoptosis. Conclusions: The study shows that the gene expression patterns of IBD, CD in particular, are quite different from those of infectious colitis, highlighting distinctive expression of genes and pathways in UC and CD. (Inflamm Bowel Dis 2007) |
| Databáze: | OpenAIRE |
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