The ribosomal RNA repeats are non-identical and directly oriented in the chloroplast genome of the red alga Porphyra purpurea
| Autor: | Michael Reith, Janet Munholland |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Chloroplasts
Porphyra purpurea Molecular Sequence Data Restriction Mapping Genome Polymerase Chain Reaction Restriction fragment Restriction map Genetics Direct repeat Ribosomal DNA Repetitive Sequences Nucleic Acid Recombination Genetic biology Base Sequence food and beverages General Medicine DNA Ribosomal RNA Molecular biology Biological Evolution Blotting Southern Chloroplast DNA RNA Ribosomal Rhodophyta biology.protein |
| Zdroj: | Current genetics. 24(5) |
| ISSN: | 0172-8083 |
| Popis: | A detailed restriction map of the chloroplast genome of the red alga Porphyra purpurea has been constructed. Southern hybridization experiments with cloned or gel-purified restriction fragments and PCR products indicate that the P. purpurea chloroplast genome is approximately 188 kb in size. This circular molecule contains two rRNA-encoding repeats (approximately 4.9 kb) that separate the genome into single-copy regions of 34 kb and 144 kb. Interestingly, these repeats are arranged in a direct orientation. In addition, DNA sequencing of the ends of both repeats revealed that the two rRNA repeats are not identical. No intramolecular recombination between the repeats can be detected. We discuss the possibility that the chloroplast genome of P. purpurea is organized like that of the ancestral chloroplast. |
| Databáze: | OpenAIRE |
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