Oxidative stress in patients with advanced CKD and renal replacement therapy: the key role of peripheral blood leukocytes
| Autor: | Claudia Yuste, Enrique Morales, Paula Jara Caro, Carmen Vida, Julia Carracedo, Gemma Valera, Noemí Ceprián, Ignacio González de Pablos, Carlos Oliva |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Antioxidant Nefrología y urología Physiology medicine.medical_treatment polymorphonuclear leukocytes Clinical Biochemistry 030232 urology & nephrology Oxidative phosphorylation RM1-950 medicine.disease_cause Fisiología Biochemistry Article mononuclear leukocytes Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine oxidative stress Xanthine oxidase Molecular Biology chemistry.chemical_classification hemodialysis biology business.industry Glutathione peroxidase Cell Biology Glutathione Malondialdehyde 030104 developmental biology Endocrinology chemistry peritoneal dialysis biology.protein Therapeutics. Pharmacology business Oxidative stress chronic kidney disease |
| Zdroj: | E-Prints Complutense. Archivo Institucional de la UCM instname Antioxidants Antioxidants, Vol 10, Iss 1155, p 1155 (2021) Volume 10 Issue 7 |
| Popis: | Oxidative stress plays a key role in the pathophysiology of chronic kidney disease (CKD). Most studies have investigated peripheral redox state focus on plasma, but not in different immune cells. Our study analyzed several redox state markers in plasma and isolated peripheral polymorphonuclear (PMNs) and mononuclear (MNs) leukocytes from advanced-CKD patients, also evaluating differences of hemodialysis (HD) and peritoneal dialysis (PD) procedures. Antioxidant (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH)) and oxidant parameters (xanthine oxidase (XO), oxidized glutathione (GSSG), malondialdehyde (MDA)) were assessed in plasma, PMNs and MNs from non-dialysis-dependent-CKD (NDD-CKD), HD and PD patients and healthy controls. Increased oxidative stress and damage were observed in plasma, PMNs and MNs from NDD-CKD, HD and PD patients (increased XO, GSSG and MDA decreased SOD, CAT, GPX and GSH altered GSSG/GSH balance). Several oxidative alterations were more exacerbated in PMNs, whereas others were only observed in MNs. Dialysis procedures had a positive effect on preserving the GSSG/GSH balance in PMNs. Interestingly, PD patients showed greater oxidative stress than HD patients, especially in MNs. The assessment of redox state parameters in PMNs and MNs could have potential use as biomarkers of the CKD progression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|