Thermo-sensitive gellan maleate/N-isopropylacrylamide hydrogels: initial 'in vitro' and 'in vivo' evaluation as ocular inserts

Autor: Marcel Popa, Mihaela Hamcerencu, Gérard Riess, Jacques Desbrières
Přispěvatelé: Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), 'Gheorghe Asachi' Technical University of Iaşi - Faculty of Chemical Engineering and Environmental Protection, Department of Natural Synthetic Polymers, Laboratoire de Photochimie et d'Ingénierie Macromoléculaires (LPIM), Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Agence Universitaire de la Francophonie
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Polymer Bulletin
Polymer Bulletin, Springer Verlag, 2020, 77, pp.741-755. ⟨10.1007/s00289-019-02772-5⟩
ISSN: 0170-0839
1436-2449
DOI: 10.1007/s00289-019-02772-5⟩
Popis: International audience; Hydrogels are among the most used polymer–drug systems, polysaccharide-based materials presenting many advantages to be used for controlled release applications. Gellan maleate/NIPAm hydrogels are hydrophilic swelling matrixes that were originally developed for biomedical applications as controlled release dosage forms. The purpose of this study is to explore the ability of gellan maleate/NIPAm thermoreversible hydrogels for ophthalmic applications as ocular inserts. Studied hydrogels were synthesized by free radical grafting/polymerization of unsaturated esters (gellan maleate) with N-isopropylacrylamide (NIPAm) and N,N′-methylenebisacrylamide as cross-linker. They are evaluated for swelling degree, in vitro inclusion and release of drugs (adrenaline and chloramphenicol) and in vivo biocompatibility. The hydrogel is shown to be a temperature-responsive material with a LCST close to 36 °C. The swelling properties and the release of biologically active compounds occur when polymer conformation changes determining phase transition at LCST. Gellan-based hydrogels present reproducible responses to alternation between ambient temperature and human body one. The stability and biocompatibility were evaluated in vitro and in vivo. Tissue response to implantation of hydrogel was determined by histological analysis (haematoxylin–eosin–methylene blue and periodic acid–Schiff). A thin fibrous capsule was observed around the implanted hydrogels. No necrosis, calcification and acute inflammatory reaction were noted. Successful preliminary results were obtained for ophthalmic applications.
Databáze: OpenAIRE
Externí odkaz: