Cloning of a gene required for tryptophan biosynthesis from Leptospira biflexa serovar patoc into Escherichia coli
| Autor: | David B. Yelton, Nyles W. Charon |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
DNA
Recombinant Molecular cloning medicine.disease_cause Microbiology Plasmid Gene bank Escherichia coli Genetics medicine Cloning Molecular Gene Leptospira Cloning biology Tryptophan Chromosome Mapping Nucleic Acid Hybridization Drug Resistance Microbial General Medicine biology.organism_classification Enterobacteriaceae Molecular biology PBR322 Genes Bacterial Transformation Bacterial Plasmids |
| Zdroj: | Gene. 28:147-152 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/0378-1119(84)90251-8 |
| Popis: | A clone bank, consisting of approx. 8100 colonies, has been created for the spirochete Leptospira biflexa serovar patoc in Escherichia coli using pBR322 as the vector. One of these clones contains the genetic information needed to complement a defect in the trpE gene of E. coli . The information resides on a 20.5-kb plasmid designated pYCl, which carries a 16-kb insert consisting of three Hind III fragments. It does not complement defects in other genes needed for the biosynthesis of tryptophan in E. coli . |
| Databáze: | OpenAIRE |
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