Extensive nuclear gyration and pervasive non-genic transcription during primordial germ cell development in zebrafish

Autor: Stefan Redl, Stefanie Möckel, Maria Mendez-Lago, Willi Salvenmoser, René F. Ketting, Edoardo Caspani, António Miguel de Jesus Domingues
Rok vydání: 2020
Předmět:
endocrine system
RNA
Untranslated
Transcription
Genetic
Zygote
Piwi-interacting RNA
piRNA
Biology
03 medical and health sciences
0302 clinical medicine
Gyration
Transcription (biology)
Primordial germ cell
medicine
Animals
RNA
Small Interfering
Molecular Biology
Zebrafish
Gene
030304 developmental biology
Cell Nucleus
Nuage
0303 health sciences
Gonadal ridge
urogenital system
Nuclear morphology
Gene Expression Regulation
Developmental
DNA-Directed RNA Polymerases
Zygotic activation
Zebrafish Proteins
biology.organism_classification
Chromatin
Cell biology
Up-Regulation
medicine.anatomical_structure
Germ Cells
Genetic Loci
Fertilization
Mutation
Intergenic transcription
DNA Transposable Elements
DNA
Intergenic
030217 neurology & neurosurgery
Germ cell
Biogenesis
Developmental Biology
Research Article
Zdroj: Development (Cambridge, England)
article-version (VoR) Version of Record
ISSN: 1477-9129
Popis: Primordial germ cells (PGCs) are the precursors of germ cells, which migrate to the genital ridge during early development. Relatively little is known about PGCs after their migration. We studied this post-migratory stage using microscopy and sequencing techniques, and found that many PGC-specific genes, including genes known to induce PGC fate in the mouse, are only activated several days after migration. At this same time point, PGC nuclei become extremely gyrated, displaying general broad opening of chromatin and high levels of intergenic transcription. This is accompanied by changes in nuage morphology, expression of large loci (PGC-expressed non-coding RNA loci, PERLs) that are enriched for retro-transposons and piRNAs, and a rise in piRNA biogenesis signatures. Interestingly, no nuclear Piwi protein could be detected at any time point, indicating that the zebrafish piRNA pathway is fully cytoplasmic. Our data show that the post-migratory stage of zebrafish PGCs holds many cues to both germ cell fate establishment and piRNA pathway activation.
Highlighted Article: In contrast to common thinking, zebrafish primordial germ cells do not rest after arrival at the genital ridge, but display many morphological changes accompanied by pervasive intergenic transcription and piRNA activation.
Databáze: OpenAIRE
Externí odkaz: