Serological evidence for Japanese encephalitis and West Nile virus infections in domestic birds in Cambodia
| Autor: | Heidi Auerswald, Anne-Sophie Ruget, Helena Ladreyt, Saraden In, Sokthearom Mao, San Sorn, Sothyra Tum, Veasna Duong, Philippe Dussart, Julien Cappelle, Véronique Chevalier |
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| Přispěvatelé: | Unité de Virologie / Virology Unit [Phnom Penh], Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Unité d'Épidémiologie et de Santé Publique [Phnom Penh], Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université Paris-Est (UPE), Ministry of Agriculture, Forestry and Fisheries [Cambodia], Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), This work was partial funded by ComAcross project and the Virology Unit of Institut Pasteur du Cambodge. The postdoctoral fellowship of HA was supported by the Calmette and Yersin Programme of the Institut Pasteur Department of International Affairs. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
040301 veterinary sciences
viruses serology L73 - Maladies des animaux Virus Volaille Serology Sérologie 0403 veterinary science 03 medical and health sciences medicine Seroprevalence domestic birds Surveillance épidémiologique 030304 developmental biology Original Research 2. Zero hunger 0303 health sciences Hemagglutination assay lcsh:Veterinary medicine biology General Veterinary Transmission (medicine) virus du Nil occidental poultry virus diseases 04 agricultural and veterinary sciences Japanese encephalitis medicine.disease biology.organism_classification Virology 3. Good health Flavivirus Japanese encephalitis virus Culicidae [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Virus encéphalite japonaise Enzootic lcsh:SF600-1100 Veterinary Science Cambodia West Nile virus |
| Zdroj: | Frontiers in Veterinary Science Frontiers in Veterinary Science, Vol 7 (2020) Frontiers in Veterinary Science, Frontiers Media, 2020, 7, pp.15. ⟨10.3389/fvets.2020.00015⟩ |
| ISSN: | 2297-1769 |
| DOI: | 10.3389/fvets.2020.00015⟩ |
| Popis: | International audience; Mosquito-borne flaviviruses with an enzootic transmission cycle like Japanese encephalitis virus (JEV) and West Nile virus (WNV) are a major public health concern. The circulation of JEV in Southeast Asia is well-documented, and the important role of pigs as amplification hosts for the virus is long known. The influence of other domestic animals especially poultry that lives in high abundance and close proximity to humans is not intensively analyzed. Another understudied field in Asia is the presence of the closely related WNV. Such analyses are difficult to perform due to the intense antigenic cross-reactivity between these viruses and the lack of suitable standardized serological assays. The main objective of this study was to assess the prevalence of JEV and WNV flaviviruses in domestic birds, detailed in chickens and ducks, in three different Cambodian provinces. We determined the flavivirus seroprevalence using an hemagglutination inhibition assay (HIA). Additionally, we investigated in positive samples the presence of JEV and WNV neutralizing antibodies (nAb) using foci reduction neutralization test (FRNT). We found 29% (180/620) of the investigated birds positive for flavivirus antibodies with an age-depended increase of the seroprevalence (OR = 1.04) and a higher prevalence in ducks compared to chicken (OR = 3.01). Within the flavivirus- positive birds, we found 43% (28/65) with nAb against JEV. We also observed the expected cross-reactivity between JEV and WNV, by identifying 18.5% double-positive birds that had higher titers of nAb than single-positive birds. Additionally, seven domestic birds (10.7%) showed only nAb against WNV and no nAb against JEV. Our study provides evidence for an intense JEV circulation in domestic birds in Cambodia, and the first serological evidence for WNV presence in Southeast Asia since decades. These findings mark the need for a re-definition of areas at risk for JEV and WNV transmission, and the need for further and intensified surveillance of mosquito-transmitted diseases in domestic animals. |
| Databáze: | OpenAIRE |
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