Metabolomics analysis of serum reveals the effect of Danggui Buxue Tang on fatigued mice induced by exhausting physical exercise
| Autor: | Bing-Kun Xiao, Sufang Shui, Xiao-Yao Miao, Jian-Yun Yang, Rong-Qing Huang, Junxing Dong |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Angelica sinensis Clinical Biochemistry Pharmaceutical Science Glyoxylate and dicarboxylate metabolism Phenylalanine Traditional Chinese medicine Pharmacology Models Biological Gas Chromatography-Mass Spectrometry Analytical Chemistry Serine 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Metabolomics Physical Conditioning Animal Drug Discovery Animals Humans Least-Squares Analysis Medicine Chinese Traditional Spectroscopy Fatigue biology biology.organism_classification Citric acid cycle Disease Models Animal 030104 developmental biology chemistry Glycine 030217 neurology & neurosurgery Biomarkers Software Drugs Chinese Herbal Signal Transduction |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 151 |
| ISSN: | 1873-264X |
| Popis: | Danggui Buxue Tang (DBT), believed to invigorate 'Qi' (vital energy) and nourish 'Blood' (body circulation), is a traditional Chinese medicine formula. In this study, a metabolomics approach with gas chromatography coupled to mass spectrometry combined with pattern recognition was adopted to investigate the underlying mechanism of the antifatigue effect of DBT on fatigue of mice induced by weight-loaded forced swimming. Fourteen endogenous metabolites, up-regulated or down-regulated, were identified in the model mice by analysis tools of partial least-squares discriminant analysis (PLS-DA) and XCMS online software. Furthermore, the metabolites were reversed by DBT treatment, offering evidence for the antifatigue effect. In addition, intervention of DBT changed the levels of biochemical parameters. DBT showed obvious efficacy on the fatigued mice possibly by regulating the pathways of phenylalanine, tyrosine and tryptophan metabolism, glycine, serine, and threonine metabolism, glyoxylate and dicarboxylate metabolism, pyruvate metabolism, and TCA cycle. This study demonstrated that DBT has a good antifatigue effect and that metabolomics is a powerful means to gain insights into the therapeutic effect of traditional Chinese medicine formulas. |
| Databáze: | OpenAIRE |
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