Evolution of Hemoglobin Genes in a Subterranean Rodent Species (Lasiopodomys mandarinus)
| Autor: | Shiming Gu, Denghui Liu, Hong Sun, Kaihong Ye, Zhenlong Wang, Dan Pan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Rodent Pseudogene hemoglobin gene complex mixtures 010603 evolutionary biology 01 natural sciences Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Mandarin vole positive selection biology.animal Brandt’s vole Gene cluster Gene lcsh:QH301-705.5 Genetics General Immunology and Microbiology biology Phylogenetic tree hypoxia biology.organism_classification 030104 developmental biology lcsh:Biology (General) Lasiopodomys mandarinus Vole General Agricultural and Biological Sciences Oxygen binding |
| Zdroj: | Biology, Vol 9, Iss 106, p 106 (2020) Biology Volume 9 Issue 5 |
| ISSN: | 2079-7737 |
| Popis: | The Mandarin vole (Lasiopodomys mandarinus), a typical subterranean rodent, has undergone hematological adaptations to tolerate the hypoxic/hypercapnic underground environment. Hemoglobin (Hb) genes encode respiratory proteins functioning principally in oxygen binding and transport to various tissues and organs. To investigate the evolution of &alpha and &beta hemoglobin (Hb) in subterranean rodent species, we sequenced Hb genes of the Mandarin vole and the related aboveground Brandt&rsquo s vole (L. brandtii). Sequencing showed that in both voles, &alpha globin was encoded by a cluster of five functional genes in the following linkage order: HBZ, HBA-T1, HBQ-T1, HBA-T2, and HBQ-T2 among these, HBQ-T2 is a pseudogene in both voles. The &beta globin gene cluster in both voles also included five functional genes in the following linkage order: HBE, HBE/HBG, HBG, HBB-T1, and HBB-T2. Phylogenetic analysis revealed that the Mandarin vole underwent convergent evolution with its related aboveground species (Brandt&rsquo s vole) but not with other subterranean rodent species. Selection pressure analyses revealed that &alpha globin genes are under strong purifying selection (&omega < 1), and branch-site analyses identified positive selection sites on HBAT-T1 and HBB-T1 in different subterranean rodent species. This suggests that the adaptive evolution of these genes enhanced the ability of Hb to store and transport oxygen in subterranean rodent species. Our findings highlight the critical roles of Hb genes in the evolution of hypoxia tolerance in subterranean rodent species. |
| Databáze: | OpenAIRE |
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