Deciphering the genetic control of fruit texture in apple by multiple family-based analysis and genome-wide association
| Autor: | Nicola Busatto, Thomas Letschka, Fabrizio Costa, Alice Tadiello, Richard G. F. Visser, Luca Bianco, Lidia Lozano, L. Poles, Eric van de Weg, Marco C. A. M. Bink, Mario Di Guardo, Walter Guerra |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Candidate gene Genotype Physiology In silico Quantitative Trait Loci SNP Single-nucleotide polymorphism Genome-wide association study Plant Science Quantitative trait locus Biology Bayesian statistics 01 natural sciences Texture (geology) RT–qPCR PBR Biodiversiteit en Genetische Variatie 03 medical and health sciences Laboratorium voor Plantenveredeling Selection (genetic algorithm) Genetics Genome-wide association study (GWAS) fungi RT-qPCR Apple food and beverages Pedigree-based analysis (PBA) Ripening Fruit texture High-resolution phenotyping PE&RC Plant Breeding Settore AGR/07 - GENETICA AGRARIA Phenotype 030104 developmental biology Fruit Malus Multigene Family EPS PBR Biodiversity and genetic variation Genome-Wide Association Study Research Paper 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany 68 (2017) 7 Journal of Experimental Botany, 68(7), 1451-1466 Journal of Experimental Botany |
| ISSN: | 0022-0957 |
| Popis: | Highlight A distinct set of QTLs related to mechanical and acoustic fruit texture features were identified in apple. Through a GWAS approach, the specific genetic control of these subtraits was elucidated. Fruit texture is a complex feature composed of mechanical and acoustic properties relying on the modifications occurring in the cell wall throughout fruit development and ripening. Apple is characterized by a large variation in fruit texture behavior that directly impacts both the consumer’s appreciation and post-harvest performance. To decipher the genetic control of fruit texture comprehensively, two complementing quantitative trait locus (QTL) mapping approaches were employed. The first was represented by a pedigree-based analysis (PBA) carried out on six full-sib pedigreed families, while the second was a genome-wide association study (GWAS) performed on a collection of 233 apple accessions. Both plant materials were genotyped with a 20K single nucleotide polymorphism (SNP) array and phenotyped with a sophisticated high-resolution texture analyzer. The overall QTL results indicated the fundamental role of chromosome 10 in controlling the mechanical properties, while chromosomes 2 and 14 were more associated with the acoustic response. The latter QTL, moreover, showed a consistent relationship between the QTL-estimated genotypes and the acoustic performance assessed among seedlings. The in silico annotation of these intervals revealed interesting candidate genes potentially involved in fruit texture regulation, as suggested by the gene expression profile. The joint integration of these approaches sheds light on the specific control of fruit texture, enabling important genetic information to assist in the selection of valuable fruit quality apple varieties. |
| Databáze: | OpenAIRE |
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