Catalase and NO CATALASE ACTIVITY1 Promote Autophagy-Dependent Cell Death in Arabidopsis

Autor:	Stig U. Andersen, Katrien Van Der Kelen, Morten Petersen, Trine Juul, Simon Bressendorff, Anna Małolepszy, Sonia Gwizdz, Frank Van Breusegem, Andrea Lorentzen, Jan-Elo Jørgensen, Daniel Hofius, Aija Auzina, Kåre Lehmann Nielsen, Svend Dam, Peter Roepstorff, Thomas Hackenberg
Jazyk:	angličtina
Rok vydání:	2013
Předmět:	chemistry.chemical_classification Hypersensitive response Reactive oxygen species Programmed cell death biology Autophagy Mutant Cell Biology Plant Science medicine.disease_cause biology.organism_classification chemistry Biochemistry Catalase Arabidopsis biology.protein medicine Oxidative stress
Zdroj:	Hackenberg, T, Juul, T, Auzina, A, Gwizdz, S, Malolepszy, A, Van Der Kelen, K, Dam, S, Bressendorff, S, Lorentzen, A M, Roepstorff, P, Lehmann Nielsen, K, Jørgensen, J-E, Hofius, D, Van Breusegem, F, Petersen, M & Andersen, S U 2013, ' Catalase and NO CATALASE ACTIVITY1 Promote Autophagy-Dependent Cell Death in Arabidopsis ', Plant Cell, vol. 25, no. 11, pp. 4616-4626 . https://doi.org/10.1105/tpc.113.117192 Hackenberg, T, Juul, T M, Auzina, A, Gwizdz, S, Malolepszy, A, Van Der Kelen, K, Dam, S S, Bressendorff, S, Lorentzen, A, Roepstorff, P, Lehmann Nielsen, K, Jørgensen, J-E, Hofius, D, Breusegem, F V, Petersen, M & Andersen, S U 2013, ' Catalase and NO CATALASE ACTIVITY1 Promote Autophagy-Dependent Cell Death in Arabidopsis ', Plant Cell, vol. 25, no. 11, pp. 4616-4626 . https://doi.org/10.1105/tpc.113.117192 Hackenberg, T, Andersen, T J, Auzina, A, Gwizdz, S, Malolepszy, A, Van Der Kelen, K, Dam, S S, Bressendorff, S, Lorentzen, A M, Roepstorff, P, Nielsen, K L, Jørgensen, J-E, Hofius, D, Van Breusegem, F, Petersen, M & Andersen, S U 2013, ' Catalase and NO CATALASE ACTIVITY1 promote autophagy-dependent cell death in Arabidopsis ', Plant Cell, vol. 25, no. 11, pp. 4616-4626 . https://doi.org/10.1105/tpc.113.117192
DOI:	10.1105/tpc.113.117192
Popis:	Programmed cell death often depends on generation of reactive oxygen species, which can be detoxified by antioxidative enzymes, including catalases. We previously isolated catalase-deficient mutants (cat2) in a screen for resistance to hydroxyurea-induced cell death. Here, we identify an Arabidopsis thaliana hydroxyurea-resistant autophagy mutant, atg2, which also shows reduced sensitivity to cell death triggered by the bacterial effector avrRpm1. To test if catalase deficiency likewise affected both hydroxyurea and avrRpm1 sensitivity, we selected mutants with extremely low catalase activities and showed that they carried mutations in a gene that we named NO CATALASE ACTIVITY1 (NCA1). nca1 mutants showed severely reduced activities of all three catalase isoforms in Arabidopsis, and loss of NCA1 function led to strong suppression of RPM1-triggered cell death. Basal and starvation-induced autophagy appeared normal in the nca1 and cat2 mutants. By contrast, autophagic degradation induced by avrRpm1 challenge was compromised, indicating that catalase acted upstream of immunity-triggered autophagy. The direct interaction of catalase with reactive oxygen species could allow catalase to act as a molecular link between reactive oxygen species and the promotion of autophagy-dependent cell death.
Databáze:	OpenAIRE
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