Construction of an intraspecific integrated linkage map of pepper using molecular markers and doubled-haploid progenies

Autor: Edmond Pochard, Alain Palloix, Véronique Lefebvre, Carole Caranta
Přispěvatelé: ProdInra, Migration, Station d'amélioration des plantes maraîchères, Institut National de la Recherche Agronomique (INRA)
Předmět:
Zdroj: HAL
Genome
Genome, NRC Research Press, 1995, pp.112-121
ISSN: 0831-2796
1480-3321
Popis: An integrated molecular linkage map of pepper (Capsicum annuum L.), including mainly RFLP and RAPD markers, has been constructed by alignment of three intraspecific linkage maps generated by segregating doubled-haploid progenies. A total of 85 markers covered approximately 820 cM in 14 linkage groups. Four linkage groups were assigned to 4 chromosomes. Two new genes of agronomic interest were located: L controlling hypersensitive resistance to TMV and up controlling the erect habit of the fruits. The C gene controlling the fruit pungency was more precisely located. This map is estimated to represent from 36 to 59% of the total pepper genome. An examination of segregation data has revealed several genomic regions with aberrant segregation ratios often favouring the agronomic big-fruited parents, particularly in crosses involving the exotic parent CM334, suggesting that these genome regions are subjected to selection during the process of doubled-haploid production. The suitability of doubled-haploid progenies for mapping projects and the differences observed between this intraspecific integrated map with earlier published interspecific pepper maps are discussed.Key words: Capsicum annuum, RFLP, RAPD, integrated linkage map, doubled-haploid progenies.
Databáze: OpenAIRE
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