Rheological properties, biocompatibility and in vivo performance of new hydrogel-based bone fillers
| Autor: | Paolo Fattori, Rodolfo Quarto, Luciano Zardi, Paolo Giannoni, Cinzia Cordazzo, Federico Villa, Mauro Fiorini |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Bone sialoprotein Biocompatibility in vivo performance biocompatibility bone fillers hydrogel Biomedical Engineering Biocompatible Materials Polyethylene glycol Bone and Bones Hydrogel Polyethylene Glycol Dimethacrylate Cell Line 03 medical and health sciences chemistry.chemical_compound Animals Humans General Materials Science Bone regeneration biology Demineralized bone matrix Chemistry dBm Mesenchymal Stem Cells 030104 developmental biology Methyl cellulose Bone Substitutes Biophysics biology.protein Osteocalcin Rabbits Rheology |
| Popis: | Three different heterologous substitutes for bone regeneration, manufactured with equine-derived cortical powder (CP), cancellous chips (CC) and demineralized bone matrix granules (DBM), were compared in in vitro and in vivo settings. We tested: a commercially available bone paste (Osteoplant-Activagen™, consisting of aqueous collagenous carrier, CP, DBM; named A); a second-generation injectable paste (20 kDa polyethylene glycol/hydroxypropyl-methyl cellulose-based hydrogel, CP, DBM; B); a pre-formed bone filler (400 kDa polyethylene oxide/hydroxypropyl-methyl cellulose-based hydrogel, CP, CC, DBM; C). Vitamin C acted as a visco-modulator during C and B β-rays sterilization, modifying graft injectability. For each filler, we examined dissolution in culture medium, gene expression of the substitute-exposed osteogenically-induced human bone marrow stromal cells (hBMSC), and performance in a rabbit bone defect model. A dissolved after 1 h, while fragmentation of B peaked after 8 h. C remained unaltered for 2 days, but affected the microenvironmental pH, slowing the proliferation of exposed cells. B-exposed hBMSC overexpressed bone sialoprotein, osteocalcin and RUNX2. For all fillers histological results evidenced bridged lesion margins, marrow replenishment and bone-remodeling. However, B-treated lesions displayed a metachromatic type II collagen-rich matrix with prehypertrophic-like cells, matching the in vitro expression of cartilage-specific markers, and suggesting a possible application of B/C double-layer monolithic osteochondral plugs for full-thickness articular defects. |
| Databáze: | OpenAIRE |
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