Constitutive cyclic GMP accumulation in Arabidopsis thaliana compromises systemic acquired resistance induced by an avirulent pathogen by modulating local signals
| Autor: | Jian Chen, Paola Fincato, Sara Cimini, W. Sabetta, Smrutisanjita Behera, Vittoria Locato, Elodie Vandelle, Laura De Gara, Alex Costa, Mabrouk Bouneb, Raman Pachaiappan, Alexander Graf, Jamshaid Hussain, Silvia Martínez-Jaime, Francesca Griggio, Diana Bellin, Maria Concetta de Pinto, Emanuela Blanco |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Proteome Transgene Arabidopsis Pseudomonas syringae Ascorbic Acid Plant disease resistance 01 natural sciences 03 medical and health sciences Bacterial Proteins cyclic GMP Gene Expression Regulation Plant Gene expression Arabidopsis thaliana Animals proteomic Disease Resistance Regulation of gene expression plant defense responses Multidisciplinary biology food and beverages transcriptomic cGMP systemic acquired resistance biology.organism_classification Ascorbic acid Plants Genetically Modified Virology Glutathione Corrigenda Cell biology Rats cGMP Plant Leaves 030104 developmental biology avirulent pathogen Guanylate Cyclase systemic acquired resistance Transcriptome Systemic acquired resistance 010606 plant biology & botany |
| Zdroj: | Scientific reports (Nature Publishing Group) 6 (2016). doi:10.1038/srep36423 info:cnr-pdr/source/autori:Hussain J, Chen J, Locato V, Sabetta W, Behera S, Cimini S, Griggio F, Martínez-Jaime S, Graf A, Bouneb M, Pachaiappan R, Fincato P, Blanco E, Costa A, De Gara L, Bellin D, de Pinto MC, Vandelle E./titolo:Constitutive cyclic GMP accumulation in Arabidopsis thaliana compromises systemic acquired resistance induced by an avirulent pathogen by modulating local signals./doi:10.1038%2Fsrep36423/rivista:Scientific reports (Nature Publishing Group)/anno:2016/pagina_da:/pagina_a:/intervallo_pagine:/volume:6 Scientific Reports |
| DOI: | 10.1038/srep36423 |
| Popis: | The infection of Arabidopsis thaliana plants with avirulent pathogens causes the accumulation of cGMP with a biphasic profile downstream of nitric oxide signalling. However, plant enzymes that modulate cGMP levels have yet to be identified, so we generated transgenic A. thaliana plants expressing the rat soluble guanylate cyclase (GC) to increase genetically the level of cGMP and to study the function of cGMP in plant defence responses. Once confirmed that cGMP levels were higher in the GC transgenic lines than in wild-type controls, the GC transgenic plants were then challenged with bacterial pathogens and their defence responses were characterized. Although local resistance was similar in the GC transgenic and wild-type lines, differences in the redox state suggested potential cross-talk between cGMP and the glutathione redox system. Furthermore, large-scale transcriptomic and proteomic analysis highlighted the significant modulation of both gene expression and protein abundance at the infection site, inhibiting the establishment of systemic acquired resistance. Our data indicate that cGMP plays a key role in local responses controlling the induction of systemic acquired resistance in plants challenged with avirulent pathogens. |
| Databáze: | OpenAIRE |
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