Phenotypic and functional differences between senescent and aged murine microglia
| Autor: | Regine Heller, Tzvetanka Bondeva, Otto W. Witte, Quratul Ain, Christian Schmeer, Milan Stojiljkovic |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Senescence Aging Telomerase Gene Expression Stimulation Biology 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation medicine Animals Cells Cultured Cellular Senescence Cyclin-Dependent Kinase Inhibitor p16 Cell Proliferation Innate immune system Microglia General Neuroscience Brain Telomere beta-Galactosidase Phenotype Up-Regulation Cell biology Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure nervous system Neurology (clinical) Geriatrics and Gerontology 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Neurobiology of Aging. 74:56-69 |
| ISSN: | 0197-4580 |
| DOI: | 10.1016/j.neurobiolaging.2018.10.007 |
| Popis: | Microglia, the key innate immune cells in the brain, have been reported to drive brain aging and neurodegenerative disorders; however, few studies have analyzed microglial senescence and the impact of aging on the properties of microglia. In the present study, we characterized senescence- and aging-associated phenotypes of murine brain microglia using well-accepted markers, including telomere length, telomerase activity, expression of p16INK4a, p21, p53, senescence-associated β-galactosidase, and a senescence-associated secretory phenotype. Quantitative real-time polymerase chain reaction analysis and a Telomeric Repeat Amplification Protocol assay indicated shortened telomeres and increased telomerase activity in senescent microglia, whereas telomeres remained unaltered and telomerase activity was reduced in aged microglia. Senescent microglia upregulated p16INK4a, p21, and p53, whereas acutely isolated microglia from the aged brain only exhibited a modest upregulation of p16INK4a. Senescent microglia showed decreased proliferation, while it was unchanged in aged microglia. Furthermore, microglia at late passages strongly upregulated expression of the senescent marker senescence-associated β-galactosidase. Senescent and aged microglia exhibited differential activation profiles and altered responses to stimulation. We conclude that microglia from the aged mouse brain do not show typical senescent changes because their phenotype and functional response strongly differ from those of senescent microglia in vitro. |
| Databáze: | OpenAIRE |
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