Docetaxel and 5-FU enhanced the inhibitory effects of apatinib and ramucirumab on growth and migration of gastric cancer
| Autor: | Jia-Qi Wu, Ruo-Yue Fan, Jing Zhai, Chong-Yong Li, Ping Wei, Li-Zong Shen, Ming-Fang He, Ping Wang, Xin-En Huang |
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| Rok vydání: | 2021 |
| Předmět: |
Embryo
Nonmammalian Pyridines Angiogenesis Inhibitors General Medicine Docetaxel Antibodies Monoclonal Humanized Vascular Endothelial Growth Factor Receptor-2 Xenograft Model Antitumor Assays General Biochemistry Genetics and Molecular Biology Animals Genetically Modified Cell Movement Stomach Neoplasms Cell Line Tumor Antineoplastic Combined Chemotherapy Protocols Animals Humans Fluorouracil General Pharmacology Toxicology and Pharmaceutics Proto-Oncogene Proteins c-akt Zebrafish Cell Proliferation |
| Zdroj: | Life sciences. 296 |
| ISSN: | 1879-0631 |
| Popis: | Gastric cancer (GC) is one of the most common malignant tumors in the world. The clinical benefit of anti-angiogenic strategy as a single drug is limited. Some studies showed that the combination of anti-angiogenic therapy and chemotherapy exhibited synergistic effect and reduced the side effects of chemotherapy drugs. We investigated the combined effects of these two types of drugs in gastric cancer cells in vitro and in vivo.cell viability, migration, invasion, and apoptosis were evaluated by CCK-8, wound-healing, transwell, and Annexin V-FITC/PI assay, respectively. In vivo anti-cancer efficacy was tested for the cell proliferation and metastasis in cell line derived tumor xenograft (CDX) model and patient derived tumor xenografted (PDX) model based on Tg (fli-1: EGFP) zebrafish embryos; RESULTS: In the cell experiments, the combination of the two types of drugs could inhibit the proliferation and metastasis of gastric cancer cells and promote apoptosis through VEGFR-2/AKT/ERK1/2 signal. In the zebrafish CDX (zCDX) model and zebrafish PDX (zPDX) model, the combination of the two treatment also showed a synergistic effect in inhibiting gastric cancer cell metastasis and cell proliferation.Apatinib/ramucirumab targeted therapy combined with docetaxel or 5-fluorouracil (5-FU) may serve as an effective treatment strategy for patients with advanced gastric cancer. |
| Databáze: | OpenAIRE |
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