The effect of potential-dependent potassium uptake on membrane potential in rat brain mitochondria
| Autor: | O. V. Akopova, Malysheva Mk, I N Man'kovskaia, Vadim F. Sagach, V. I. Nosar, L I Kolchinskaia |
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| Rok vydání: | 2013 |
| Předmět: |
Membrane potential
brain mitochondria Potassium chemistry.chemical_element Depolarization Mitochondrion oxygen consumption Biochemistry Potassium channel K+ATP-channel K(+) lcsh:Biochemistry Glibenclamide Membrane repolarization chemistry transport Biophysics medicine lcsh:QD415-436 membrane potential Respiration rate medicine.drug |
| Zdroj: | Scopus-Elsevier Ukrainian Biochemical Journal, Vol 85, Iss 1, Pp 33-41 (2013) |
| ISSN: | 2409-4943 |
| Popis: | The effect of potential-dependent potassium uptake on the transmembrane potential difference (DeltaPsi(m)) in rat brain mitochondria has been studied. It was shown that in potassium concentration range of 0-120 mM the potential-dependent K(+)-uptake into matrix leads to the increase in respiration rate and mitochondrial depolarization. ATP-dependent potassium channel (K+(ATP)-channel) blockers, glibenclamide and 5-hydroxydecanoate, block approximately 35% of potential-dependent potassium uptake in the brain mitochondria. It was shown that K+(ATP)-channel blockage results in membrane repolarization by approximately 20% of control, which is consistent with experimental dependence of DeltaPsi(m) on the rate of potential-dependent potassium uptake. Obtained experimental data give the evidence that functional activity of K+(ATP)-channel is physiologically important in the regulation of membrane potential and energy-dependent processes in brain mitochondria. |
| Databáze: | OpenAIRE |
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