Proteomic Analysis of Human Small Cell Lung Cancer Tissues: Up-Regulation of Coactosin-Like Protein-1
| Autor: | Kwang-Hyung Lee, Jung-Jae Ko, Kwang-Hoe Chung, Hye-Cheol Jeong, Gwang-Il Kim, Sang Ho Cho, Jeong-Hee Yang |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Proteomics Gene isoform Lung Neoplasms Blotting Western Bronchi Respiratory Mucosa Biochemistry Metastasis Western blot Laminin Biomarkers Tumor medicine Humans Electrophoresis Gel Two-Dimensional Aged Aged 80 and over biology medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Microfilament Proteins Reproducibility of Results General Chemistry Middle Aged medicine.disease Immunohistochemistry Small Cell Lung Carcinoma Molecular biology Peptide Fragments Reverse transcriptase Up-Regulation respiratory tract diseases Real-time polymerase chain reaction Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Proteome biology.protein Female |
| Zdroj: | Journal of Proteome Research. 10:269-276 |
| ISSN: | 1535-3907 1535-3893 |
| DOI: | 10.1021/pr100714b |
| Popis: | Small cell lung cancer (SCLC) is the leading cause of cancer death, with a high propensity for aggressiveness and metastasis even in an early stage. Thus, identification of biomarkers as early diagnostics and treatment is needed. In this study, we investigated differentially regulated proteins between human SCLC tissues and normal bronchial epithelium by proteomic analysis using two-dimensional electrophoresis (2-DE) and MALDI-TOF mass spectrometry. Seven proteins and protein isoforms, including, γ-actin, tubulin α-1B, laminin B1, coactosin-like protein-1 (COTL-1), ubiquitin carboxyl-terminal esterase L1, ubiquitin-conjugating enzyme E2-25K, and carbonic anhydrase 1, were up-regulated more than 2 fold in SCLC tissues. In particular, up-regulated COTL-1 expression was validated by Western blot analysis, immunohistochemistry, and reverse transcription quantitative polymerase chain reaction (RT-qPCR). Moreover, most SCLC tissues (93%; 28/30) were COTL-1-positive in immunohistochemistry, whereas only 16% (10/64) of nonsmall cell lung cancer (NSLC) tissues were. Taken together, this SCLC proteomic data may help in establishing a human SCLC proteome database. COTL-1 may be a biomarker or a therapeutic target in SCLC patients. |
| Databáze: | OpenAIRE |
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