Cooperation between subclasses of T lymphocytes in thein vitro generation of cytotoxicity against a mutant H-2K difference An analysis with anti-Lyt antisera
| Autor: | Marian Y. van der Meulen, Paul de Greeve, C. J. M. Melief, Bernard J. Christiaans |
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| Rok vydání: | 1979 |
| Předmět: |
Cytotoxicity
Immunologic T-Lymphocytes Lymphocyte Cooperation Immunology Mutant chemical and pharmacologic phenomena Biology Mice Animals Immunology and Allergy Cytotoxic T cell Cytotoxicity Antilymphocyte Serum Lymphokine-activated killer cell H-2 Antigens hemic and immune systems Mixed lymphocyte reaction Natural killer T cell Molecular biology In vitro Killer Cells Natural Mice Inbred C57BL Mutation Lymphocyte Culture Test Mixed |
| Zdroj: | European Journal of Immunology. 9:7-12 |
| ISSN: | 1521-4141 0014-2980 |
| DOI: | 10.1002/eji.1830090103 |
| Popis: | The cellular requirements for the generation of cytotoxicity in mixed lymphocyte reaction (MLR) across an H-2K mutant difference were analyzed. Treatment of C57BL/6 lymphocytes with either anti-Lyt-1 or anti-Lyt-2 serum and complement strongly reduced their capacity to mount a cytotoxic response against the B6.C-H-2ba mutant. Almost complete reconstitution of the cytotoxic response occurred when a mixture of anti-Lyt-1 and anti-Lyt-2-treated cells was allowed to respond. Once cytotoxicity was generated, only anti-Lyt-2 serum affected it. It is concluded that in the cytotoxic response to the ba mutant, Lyt-1 helper cells collaborate with Lyt-2,3 killer cell precursors in the generation of Lyt-2,3 killer cells. Lyt-1,2,3 cells play, if any, a minor role in the generation of cytotoxicity. By comparison, the capacity to generate killer cells in MLR against an H-2K + IA difference was only affected by anti-Lyt-2 and not by anti-Lyt-1 serum, indicating that this response is relatively independent of help by Lyt-1 cells under the conditions tested. |
| Databáze: | OpenAIRE |
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