The cyclic AMP receptor of Escherichia coli: immunological studies in extracts of Escherichia coli and other organisms
| Autor: | Wayne B. Anderson, Ira Pastan |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Salmonella typhimurium
Immunodiffusion Time Factors Receptors Drug Staphylococcus ved/biology.organism_classification_rank.species Biophysics Enterobacter medicine.disease_cause Tritium Biochemistry Antigen-Antibody Reactions Species Specificity medicine Cyclic AMP Escherichia coli Animals Ultrasonics Anaerobiosis Molecular Biology Antiserum Clostridium Binding Sites biology Caulobacter crescentus ved/biology Photobacterium Plants Ouchterlony double immunodiffusion biology.organism_classification Precipitin Proteus Proteus mirabilis Aerobiosis Cyclic AMP receptors Mutation Methanosarcina barkeri Spectrophotometry Ultraviolet Rabbits Bacillus subtilis Protein Binding |
| Zdroj: | Biochimica et biophysica acta. 320(3) |
| ISSN: | 0006-3002 |
| Popis: | An antiserum specific for the cyclic adenosine 3′,5′-monophosphate receptor from Escherichia coli has been employed to detect the presence of a similar protein in cellular extracts of a number of diverse organisms. In Ouchterlony double-diffusion experiments cellular extracts from Photobacterium fisheri, Aerobacter aerogenes, Proteus mirabilis , and Salmonella typhimurium all showed precipitin bands with E. coli cyclic AMP receptor-antiserum. The extract from Caulobacter crescentus exhibited slight cross-reactivity. Similar results were obtained with an immuno-precipitation assay used to quantitate the amount of cyclic AMP receptor-like protein present. Extracts from a variety of organisms were found to bind cyclic AMP when the usual (NH 4 ) 2 SO 4 precipitation assay for cyclic AMP receptor was employed. Only the extract from Methanosarcina barkeri was inactive. Some extracts prepared from E. coli grown on Luria broth were observed to have no cyclic AMP binding activity. Antiserum was used to determine the presence of cyclic AMP receptor in these inactive extracts. These preparations usually regain binding activity on standing at 4°C for 2–3 days. |
| Databáze: | OpenAIRE |
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