Characterization of the multigene family TaHKT 2;1 in bread wheat and the role of gene members in plant Na+ and K+ status
| Autor: | Tanveer Ul-Haq, Timothy D. Colmer, H.A. Chandima K. Ariyarathna, Michael G. Francki |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
China
Transcription Genetic Pseudogene Molecular Sequence Data Plant Science Biology IWGSS Genes Plant Polymorphism Single Nucleotide Exon INDEL Mutation Species Specificity Gene Expression Regulation Plant Complementary DNA Gene expression Tissue Na+ Amino Acid Sequence Cis regulatory elements Promoter Regions Genetic Gene Tissue K+ Triticum Plant Proteins Sequence Deletion Genetics Ions Base Sequence Alternative splicing Sodium Intron Chromosome Mapping Promoter Bread Exons Sequence Analysis DNA Aneuploidy Molecular biology Introns Aneuploid lines Multigene Family Potassium Hydrophobic and Hydrophilic Interactions Pseudogenes Research Article |
| Zdroj: | BMC Plant Biology |
| ISSN: | 1471-2229 |
| Popis: | Background A member of the TaHKT2;1 multigene family was previously identified as a Na+ transporter with a possible role in root Na+ uptake. In the present study, the existing full-length cDNA of this member was used as a basis to query the International Wheat Genome Survey Sequence to identify all members of the TaHKT2;1 family. Individual TaHKT2;1 genes were subsequently studied for gene and predicted protein structures, promoter variability, tissue expression and their role in Na+ and K+ status of wheat. Results Six TaHKT2;1 genes were characterized which included four functional genes (TaHKT2;1 7AL-1, TaHKT2;1 7BL-1, TaHKT2;1 7BL-2 and TaHKT2;1 7DL-1) and two pseudogenes (TaHKT2;1 7AL-2 and TaHKT2;1 7AL-3), on chromosomes 7A, 7B and 7D of hexaploid wheat. Variability in protein domains for cation specificity and in cis-regulatory elements for salt response in gene promoters, were identified amongst the functional TaHKT2;1 members. The functional genes were expressed under low and high NaCl conditions in roots and leaf sheaths, but were down regulated in leaf blades. Alternative splicing events were evident in TaHKT2;1 7AL-1. Aneuploid lines null for each functional gene were grown in high NaCl nutrient solution culture to identify potential role of each TaHKT2;1 member. Aneuploid lines null for TaHKT2;1 7AL-1, TaHKT2;1 7BL-1 and TaHKT2;1 7BL-2 showed no difference in Na+ concentration between Chinese Spring except for higher Na+ in sheaths. The same aneuploid lines had lower K+ in roots, sheath and youngest fully expanded leaf but only under high (200 mM) NaCl in the external solution. There was no difference in Na+ or K+ concentration for any treatment between aneuploid line null for the TaHKT2;1 7DL-1 gene and Chinese Spring. Conclusions TaHKT2;1 is a complex family consisting of pseudogenes and functional members. TaHKT2;1 genes do not have an apparent role in controlling root Na+ uptake in bread wheat seedlings under experimental conditions in this study, contrary to existing hypotheses. However, TaHKT2;1 genes or, indeed other genes in the same chromosome region on 7AL, are candidates that may control Na+ transport from root to sheath and regulate K+ levels in different plant tissues. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink