Analysis of genome sequence and trehalose lipid production peculiarities of the thermotolerant Gordonia strain
| Autor: | Yanina Delegan, Kirill Petrikov, Armen S. Sargsyan, Mikhail B. Vainstein, Bella Babayan, Nelli Hovhannisyan |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
food.ingredient
Starch Gordonia Applied Microbiology and Biotechnology Actinobacteria Surface-Active Agents 03 medical and health sciences chemistry.chemical_compound Hydrolysis food Gordonia Bacterium Gene Phylogeny Soil Microbiology 030304 developmental biology 0303 health sciences biology Strain (chemistry) 030306 microbiology Temperature Trehalose General Medicine Carbohydrate biology.organism_classification Hydrocarbons Petroleum chemistry Biochemistry Genes Bacterial Glycolipids Genome Bacterial |
| Zdroj: | Journal of Basic Microbiology. 60:14-21 |
| ISSN: | 0233-111X |
| DOI: | 10.1002/jobm.201900439 |
| Popis: | Gordoniae are one of the most promising hydrocarbon-oxidizing actinobacteria. Here we present the genome sequence analysis of thermotolerant strain Gordonia sp. 1D isolated from oil-refinery soil. It is capable of alkane consumption and biosurfactant production at temperatures of up to 50°C. Gordonia sp. 1D demonstrates maximum biosurfactant production when grown on hexadecane, and at 40°C it was slightly higher than at 27°C: 35 and 39 mN/m, respectively. For the first time, it was experimentally confirmed that the carbohydrate component of extracellular biosurfactants produced by strain 1D is trehalose. In addition, genes for the production of trehalose lipid biosurfactants were identified. The genetic determinants for two different pathways for trehalose synthesis were found. The strain carries genes otsA and otsB involved in de novo trehalose biosynthesis. Moreover, the genes treY and treZ responsible for trehalose biosynthesis from maltooligosaccharides and starch or glycogen were identified. |
| Databáze: | OpenAIRE |
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