Addition of zinc methacrylate in dental polymers: MMP-2 inhibition and ultimate tensile strength evaluation
| Autor: | Flávio Fernando Demarco, Sergio Roberto Peres Line, Rodrigo Varella de Carvalho, Adriana Etges, Fabrício Aulo Ogliari, Ana Paula de Souza, Evandro Piva, Adriana Fernandes da Silva, Sandrina Henn |
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| Rok vydání: | 2011 |
| Předmět: |
Matrix metalloproteinase inhibitor
Gelatin Zymography Gingiva chemistry.chemical_element Zinc Matrix Metalloproteinase Inhibitors Matrix metalloproteinase Methacrylate Polyethylene Glycols Tissue Culture Techniques Dental Materials Mice Polymethacrylic Acids Tensile Strength Materials Testing Ultimate tensile strength Animals Zymography Enzyme Inhibitors General Dentistry Bond strength chemistry Zinc Compounds Culture Media Conditioned Matrix Metalloproteinase 2 Methacrylates Stress Mechanical Nuclear chemistry |
| Zdroj: | Clinical Oral Investigations. 16:531-536 |
| ISSN: | 1436-3771 1432-6981 |
| Popis: | This study evaluated the effect of zinc methacrylate (ZM) on the inhibition of matrix metalloproteinase 2 (MMP-2) and the ultimate tensile strength (UTS) of an experimental polymer. Enzymes secreted from mouse gingival tissues were analyzed by gelatin zymography in buffers containing 5 mM CaCl(2) (Tris-CaCl(2)) in 50 mM Tris-HCl buffer with various concentrations of ZM (0.5, 1, 2, 4, 8, and 16 mM). The matrix metalloproteinases present in the conditioned media were characterized by immunoprecipitation. The polymer UTS evaluation was performed in eight groups with various concentrations of ZM (0, 0.5, 1, 2.5, 5, 10, 20, and 30 wt.%), in a mechanical testing machine. MMP-2 (62 kDa) was detected in the zymographic assays and inhibited by ZM in all tested concentrations. UTS data were submitted to one-way ANOVA and Tukey's test (α = 0.05), and no significant differences were observed among groups, except in the polymer containing 30% ZM, presenting a significantly lower value when compared with the control group (p 0.05). The results suggest that ZM inhibits MMP-2 expression in all concentrations tested, while small concentrations did not affect the ultimate tensile strength of the polymer. Zinc methacrylate is a metalloproteinase inhibitor that can be copolymerized with other methacrylate monomers. Yet, the addition of ZM did not affect the resin bond strength. Thus, in vivo tests should be performed to evaluate the performance of this material. |
| Databáze: | OpenAIRE |
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