E-Beam Nanostructuring and Direct Click Biofunctionalization of Thiol–Ene Resist
| Autor: | Zandi Shafagh R, Alexander Vastesson, Weijin Guo, Tommy Haraldsson, van der Wijngaart W |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Nanoteknik
Fabrication Materials science General Physics and Astronomy Electrons Nanotechnology 02 engineering and technology 010402 general chemistry OSTE biohybrid e-beam nanoscale NEMS protein patterning resist thiol−ene 01 natural sciences Electron beam processing General Materials Science Sulfhydryl Compounds Nanoscopic scale Ene reaction Nanoelectromechanical systems Optical Imaging General Engineering 021001 nanoscience & nanotechnology Nanostructures 0104 chemical sciences Microscopy Fluorescence Resist Click chemistry Nano Technology Click Chemistry 0210 nano-technology Electron-beam lithography |
| Zdroj: | ACS Nano |
| ISSN: | 1936-086X 1936-0851 |
| DOI: | 10.1021/acsnano.8b03709 |
| Popis: | Electron beam lithography (EBL) is of major importance for ultraminiaturized biohybrid system fabrication, as it allows combining biomolecular patterning and mechanical structure definition on the nanoscale. Existing methods are limited by multistep biomolecule immobilization procedures, harsh processing conditions that are harmful to sensitive biomolecules, or the structural properties of the resulting protein monolayers or hydrogel-based resists. This work introduces a thiol-ene EBL resist with chemically reactive thiol groups on its native surface that allow the direct and selective "click" immobilization of biomolecules under benign processing conditions. We constructed EBL structured features of size down to 20 nm, and direct functionalized the nanostructures with a sandwich of biotin and streptavidin. The facile combination of polymer nanostructuring with biomolecule immobilization enables mechanically robust biohybrid components of interest for nanoscale biomedical, electronic, photonic, and robotic applications. QC 20181114 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|