Heterogeneous distribution of histone methylation in mature human sperm

Autor: Santiago Brugo-Olmedo, Marina Romanato, Mariano G. Buffone, Florenza A. La Spina, Rocío Melissa Rivera, Sabrina De Vincentiis, Vanina Julianelli
Rok vydání: 2013
Předmět:
Male
endocrine system
CIENCIAS MÉDICAS Y DE LA SALUD
Fisiología
Methylation
Epigenesis
Genetic
purl.org/becyt/ford/1 [https]
Ciencias Biológicas
Histones
Histone methylation
Histone H2A
Genetics
Humans
Epigenetics
Cancer epigenetics
purl.org/becyt/ford/1.6 [https]
Spermatogenesis
reproductive and urinary physiology
Genetics (clinical)
Epigenomics
EPIGENETIC
Cell Nucleus
HUMAN SPERM
biology
urogenital system
EZH2
Otras Medicina Básica
Obstetrics and Gynecology
purl.org/becyt/ford/3.1 [https]
General Medicine
Histone-Lysine N-Methyltransferase
Bioquímica y Biología Molecular
SPERM SUBPOPULATIONS
Molecular biology
Spermatozoa
Cell biology
Semen Analysis
Medicina Básica
Histone
Reproductive Medicine
Histone methyltransferase
biology.protein
Histone Methyltransferases
purl.org/becyt/ford/3 [https]
CIENCIAS NATURALES Y EXACTAS
Developmental Biology
HISTONE MODIFICATIONS
HeLa Cells
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
ISSN: 1573-7330
Popis: Purpose To analyze the presence of various histone modifications in ejaculated human spermatozoa Methods In this prospective study, seminal ejaculates from 39 normozoospermic individuals were evaluated for semen analysis and the presence of histone modifications in isolated nuclei. Results We observed heterogeneous presence of histone methylation in normal mature human sperm. We observed that 12 to 30 % of the nuclei of normal sperm contain a heterogeneous distribution of the marks H3K4Me1, H3K9Me2, H3K4Me3, H3K79Me2, and H3K36Me3. Moreover, the presence of these marks is higher in the poor motile fraction of the ejaculate, which is associated with poor morphology and functional quality. In contrast, we did not observe histone acetylation (H3K4Ac and H4K5Ac) in normal or abnormal mature human sperm Conclusions Defects in the process of spermatogenesis may alter the correct epigenetic programing in mature sperm. Further studies are required to evaluate the impact of these findings in human infertility Fil: la Spina, Florenza Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina Fil: Romanato, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina Fil: Brugo Olmedo, Santiago. Centro Médico Seremas; Argentina Fil: de Vicentiis, Sabrina. Centro Médico Seremas; Argentina Fil: Julianelli, Vanina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina Fil: Rivera, Rocío M.. University Of Missouri; Estados Unidos Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Databáze: OpenAIRE
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