Identification of the first glyphosate transporter by genomic adaptation
| Autor: | Lisa Maria Schulz, Dennis Wicke, Johannes Gibhardt, Fabian M. Commichau, Sabine Lentes, Anja Poehlein, Rolf Daniel, Patricia Scholz, Till Ischebeck |
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| Rok vydání: | 2019 |
| Předmět: |
Amino Acid Transport Systems
Acidic Mutant Glycine Bacillus subtilis medicine.disease_cause Microbiology 03 medical and health sciences chemistry.chemical_compound Glutamine synthetase Escherichia coli Aromatic amino acids medicine Ecology Evolution Behavior and Systematics 030304 developmental biology 0303 health sciences biology Herbicides 030306 microbiology Membrane Transport Proteins EPSP synthase biology.organism_classification Adaptation Physiological Enzyme Activation chemistry Glufosinate Biochemistry Symporter 3-Phosphoshikimate 1-Carboxyvinyltransferase Genome Bacterial |
| Zdroj: | Environmental Microbiology. 21:1287-1305 |
| ISSN: | 1462-2920 1462-2912 |
| DOI: | 10.1111/1462-2920.14534 |
| Popis: | The soil bacterium Bacillus subtilis can get into contact with growth-inhibiting substances, which may be of anthropogenic origin. Glyphosate is such a substance serving as a nonselective herbicide. Glyphosate specifically inhibits the 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase, which generates an essential precursor for de novo synthesis of aromatic amino acids in plants, fungi, bacteria and archaea. Inhibition of the EPSP synthase by glyphosate results in depletion of the cellular levels of aromatic amino acids unless the environment provides them. Here, we have assessed the potential of B. subtilis to adapt to glyphosate at the genome level. In contrast to Escherichia coli, which evolves glyphosate resistance by elevating the production and decreasing the glyphosate sensitivity of the EPSP synthase, B. subtilis primarily inactivates the gltT gene encoding the high-affinity glutamate/aspartate symporter GltT. Further adaptation of the gltT mutants to glyphosate led to the inactivation of the gltP gene encoding the glutamate transporter GltP. Metabolome analyses confirmed that GltT is the major entryway of glyphosate into B. subtilis. GltP, the GltT homologue of E. coli also transports glyphosate into B. subtilis. Finally, we found that GltT is involved in uptake of the herbicide glufosinate, which inhibits the glutamine synthetase. |
| Databáze: | OpenAIRE |
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