A protein interaction map for cell polarity development
| Autor: | Mark S. Longtine, Amos Mckenzie, Elizabeth Brazeau, John J. Moskow, Stanley Fields, Guang-Chao Chen, Wei Guo, Clarence S.M. Chan, Amy Hin Yan Tong, Trisha N. Davis, Laura R. Schenkman, Bryan A. Sundin, John R. Pringle, Juliane P. Caviston, Charles Boone, David G. Drubin, Becky Drees, Michelle W. Lau, Keith G. Kozminski, Peter Novick, Erfei Bi, Patrick Brennwald |
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| Rok vydání: | 2001 |
| Předmět: |
rho GTP-Binding Proteins
Saccharomyces cerevisiae Proteins Polarity (physics) Saccharomyces cerevisiae cytoskeleton Rho proteins secretion cell polarity endocytosis Biology Septin Article Fungal Proteins 03 medical and health sciences 0302 clinical medicine Bacterial Proteins Two-Hybrid System Techniques Cell polarity Cytoskeleton Actin 030304 developmental biology cdc42 GTP-Binding Protein Saccharomyces cerevisiae 0303 health sciences Secretory Vesicles Cell Polarity Cell Biology Actin cytoskeleton Actins Endocytosis Cell biology Genes cdc Luminescent Proteins Cdc42 GTP-Binding Protein 030217 neurology & neurosurgery Cytokinesis |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 0021-9525 |
| Popis: | Many genes required for cell polarity development in budding yeast have been identified and arranged into a functional hierarchy. Core elements of the hierarchy are widely conserved, underlying cell polarity development in diverse eukaryotes. To enumerate more fully the protein–protein interactions that mediate cell polarity development, and to uncover novel mechanisms that coordinate the numerous events involved, we carried out a large-scale two-hybrid experiment. 68 Gal4 DNA binding domain fusions of yeast proteins associated with the actin cytoskeleton, septins, the secretory apparatus, and Rho-type GTPases were used to screen an array of yeast transformants that express ∼90% of the predicted Saccharomyces cerevisiae open reading frames as Gal4 activation domain fusions. 191 protein–protein interactions were detected, of which 128 had not been described previously. 44 interactions implicated 20 previously uncharacterized proteins in cell polarity development. Further insights into possible roles of 13 of these proteins were revealed by their multiple two-hybrid interactions and by subcellular localization. Included in the interaction network were associations of Cdc42 and Rho1 pathways with proteins involved in exocytosis, septin organization, actin assembly, microtubule organization, autophagy, cytokinesis, and cell wall synthesis. Other interactions suggested direct connections between Rho1- and Cdc42-regulated pathways; the secretory apparatus and regulators of polarity establishment; actin assembly and the morphogenesis checkpoint; and the exocytic and endocytic machinery. In total, a network of interactions that provide an integrated response of signaling proteins, the cytoskeleton, and organelles to the spatial cues that direct polarity development was revealed. |
| Databáze: | OpenAIRE |
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