Peripheral blood and BM CD34+ CD38− cells show better resistance to cryopreservation than CD34+ CD38+ cells in autologous stem cell transplantation
| Autor: | H. Sovalat, Ph. Hénon, Mario Ojeda-Uribe, V Chabouté, A Marr, A Brunot, D. Bourderont, H Lewandowski, P Peter |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Male Cancer Research Adolescent Cell Survival Immunology Cell CD34 Antigens CD34 Cell Separation CD38 Biology Transplantation Autologous Cryopreservation Andrology Autologous stem-cell transplantation Antigens CD immune system diseases hemic and lymphatic diseases medicine Humans Immunology and Allergy Progenitor cell ADP-ribosyl Cyclase Genetics (clinical) Aged Transplantation Membrane Glycoproteins Hematopoietic stem cell hemic and immune systems Cell Biology Middle Aged Flow Cytometry Hematopoietic Stem Cells ADP-ribosyl Cyclase 1 Haematopoiesis medicine.anatomical_structure Oncology Female Stem Cell Transplantation |
| Zdroj: | Cytotherapy. 6:571-583 |
| ISSN: | 1465-3249 |
| DOI: | 10.1080/14653240410011918 |
| Popis: | We and others have shown a critical role for CD34+ CD38- cells in hematopoietic recovery after autologous stem cell transplantation (ASCT), in particular for platelet reconstitution. Thus a routine assessment of CD34+ CD38- cells in freezing-thawing procedures for autografting could represent an important tool for predicting poor engraftment.To compare the impact of cryopreservation on CD34+ CD38+ and CD34+ CD38- hematopoietic stem cell subsets, 193 autograft products collected in 84 patients with malignancies were assessed before controlled-rate cryopreservation in 10% DMSO and after thawing for autografting.Cell counts after thawing were significantly different from the pre-freezing counts for total CD34+ (P0.0001) and CD34+ CD38+ (P0.0001) cells, but not for CD34+ CD38- cells (P=0.252). Median losses for CD34+, CD34+ CD38+ and CD34+ CD38- cells were, respectively, 11.8%, 11.4% and 0.0%. The magnitude of fresh/post-thawing percentage cell variation was significantly different when comparing between the CD34+ CD38+ and CD34+ CD38- cell subsets (P0.001). Moreover, CD34+ CD38- cells exhibited recovery valuesor =100% in 85/160 graft products, compared with 51/193 in CD34+ CD38+ cells (P0.0001). Also, recovery valuesor =90% were significantly better in the CD34+ CD38- (98/160 grafts) than in the CD34+ CD38+ subsets (89/193 grafts) (P0.01).In this work we have demonstrated that CD34+ cells that do not express the CD38 Ag show a significantly better resistance to cryopreservation. This could represent another example of the particular ability of less committed progenitor cells to overcome environmental injuries. Moreover, we consider routine assessment of CD34+ CD38- cells before freezing as clinically relevant, but post-thawing controls may be avoided because of their good resistance to freezing. |
| Databáze: | OpenAIRE |
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